Šilkūnas Mantas, Bavirša Mark, Saulė Rita, Batiuškaitė Danutė, Saulis Gintautas
Department of Biology, Faculty of Natural Sciences, Vytautas Magnus University, 8 Vileikos str., LT-44404 Kaunas, Lithuania.
Department of Biology, Faculty of Natural Sciences, Vytautas Magnus University, 8 Vileikos str., LT-44404 Kaunas, Lithuania.
Bioelectrochemistry. 2021 Feb;137:107636. doi: 10.1016/j.bioelechem.2020.107636. Epub 2020 Aug 21.
Bleomycin, which is the most widely used drugs in electrochemotherapy, requires oxygen to be able to make single- or double-strand brakes in DNA. However, the concentration of oxygen in tumours can be lower than 1%. The aim of this study was to find out whether oxygen concentration in the medium in which cells loaded with bleomycin are incubated, affects the effectiveness of electrochemotherapy in vitro. Experiments were carried out on mouse hepatoma MH-22A cells. Cells were loaded with bleomycin by using a single square-wave electric pulse (2 kV/cm, 100 μs) under normoxic conditions, seeded into Petri dishes, and grown under normoxic and hypoxic conditions. Cell viability was determined by means of a colony-forming assay. We demonstrated that when cells loaded with bleomycin were incubated in hypoxia (0.2% O), up to 5.3-fold higher concentrations of bleomycin were needed to kill them in comparison with cells grown in normoxia (18.7% O).
博来霉素是电化学疗法中使用最广泛的药物，它需要氧气才能在DNA中产生单链或双链断裂。然而，肿瘤中的氧气浓度可能低于1%。本研究的目的是确定装载博来霉素的细胞所培养的培养基中的氧气浓度是否会影响体外电化学疗法的效果。实验在小鼠肝癌MH-22A细胞上进行。在常氧条件下，通过单次方波电脉冲（2 kV/cm，100 μs）使细胞装载博来霉素，接种到培养皿中，并在常氧和低氧条件下培养。通过集落形成试验测定细胞活力。我们证明，当装载博来霉素的细胞在低氧（0.2% O₂）条件下培养时，与在常氧（18.7% O₂）条件下生长的细胞相比，需要高达5.3倍的博来霉素浓度才能杀死它们。
