Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr el Aini st., Cairo 11562, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr el Aini st., Cairo 11562, Egypt.
J Chromatogr B Analyt Technol Biomed Life Sci. 2020 Nov 20;1158:122334. doi: 10.1016/j.jchromb.2020.122334. Epub 2020 Aug 26.
Jasminum is an important genus in the olive family (Oleaceae), comprising about 200 species distributed all over the world. In the current study, the methanolic extract of Jasminum grandiflorum subsp. floribundum aerial parts and its respective fractions; dichloromethane and n-butanol fractions were analyzed using ultra-performance liquid chromatography coupled to high resolution mass (UPLC-HRMS) for profiling and characterization of the plant metabolites. More than seventy metabolites were identified belonging to different classes including phenolic acids, flavonoids, secoiridoids, iridoids, lignans, fatty acids, and triterpenes. The samples were also assessed for their angiotensin-I-converting enzyme (ACE) and renin inhibitory activity along with their antioxidant potential using five complementary assays: TAC (total antioxidant capacity), DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid), FRAP (ferric reducing antioxidant power) and iron reducing power. The results revealed that the n-butanol fraction showed a potent ACE and renin inhibition as compared to Lisinopril and Aliskiren standard drugs (24.66 ± 2.41 ng/mL vs. 18.37 ± 1.21 ng/mL and 141.14 ± 5.28 ng/mL vs. 447.87 ± 3.2 ng/mL, respectively) and also a strong antioxidant activity. Interestingly, the secoiridoids, dominated metabolites detected in the n-butanol fraction, revealed the potential of them for management of the hypertension diseases. The total extract and fractions were also standardized using HPLC analysis of the major secoiridoid glycoside; oleuropein. Finally, J. grandiflorum standardized extract could be considered as a target for further studies to discover a new therapeutic anti-hypertensive drug.
素馨属是橄榄科（Oleaceae）中一个重要的属，约有 200 种，分布于世界各地。在本研究中，使用超高效液相色谱-高分辨率质谱联用（UPLC-HRMS）对素馨花（Jasminum grandiflorum subsp. floribundum）地上部分及其相应的氯仿和正丁醇部分的甲醇提取物进行了分析，以对植物代谢物进行分析和鉴定。鉴定出 70 多种代谢物，属于不同类别，包括酚酸、类黄酮、裂环环烯醚萜、环烯醚萜、木脂素、脂肪酸和三萜。还评估了样品对血管紧张素转化酶（ACE）和肾素的抑制活性以及它们的抗氧化潜力，使用了五种互补的测定方法：TAC（总抗氧化能力）、DPPH（1,1-二苯基-2-苦基肼）、ABTS（2,2'- 氮杂双-3-乙基苯并噻唑啉-6-磺酸）、FRAP（铁还原抗氧化能力）和铁还原能力。结果表明，与赖诺普利和阿利克仑标准药物相比，正丁醇部分具有更强的 ACE 和肾素抑制作用（24.66 ± 2.41 ng/mL 对 18.37 ± 1.21 ng/mL 和 141.14 ± 5.28 ng/mL 对 447.87 ± 3.2 ng/mL），同时也具有很强的抗氧化活性。有趣的是，正丁醇部分检测到的裂环环烯醚萜，是潜在的治疗高血压疾病的物质。还使用主要裂环环烯醚萜糖苷；橄榄苦苷的 HPLC 分析对总提取物和各部分进行了标准化。最后，素馨花标准化提取物可以作为进一步研究的目标，以发现一种新的治疗高血压的药物。
