Departement of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, P.O. Box 12211, Egypt.
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, El Kasr El Aini Street, Cairo, 11562, Egypt.
J Ethnopharmacol. 2021 Jul 15;275:114083. doi: 10.1016/j.jep.2021.114083. Epub 2021 Apr 6.
Jasminum grandiflorum L. is a medicinal plant widely used in the traditional system of Medicine as an anthelmintic in ringworm infections, for treating ulcers, stomatitis, skin diseases, and wounds.
The emergence of resistance by different parasites to currently used chemicals has been reported. There are increasing needs for more effective and safer parasiticides. Therefore, the current study was designed to investigate the methanolic extract of the aerial parts of J. grandiflorum subsp. Floribundum (JGTE) to confirm its traditional uses as anthelmintic through a bioassay-guided fractionation and isolation of the active components with anthelmintic activity.
The JGTE was partitioned into dichloromethane (DCM-F) and n-butanol (BuOH-F) fractions. The JGTE, fractions, and the isolated compounds were tested in vitro for their anthelmintic activity using two nematodes; one larval stage of cestode and one arthropod. Four major compounds were isolated from the most active fraction (BuOH-F) including two flavonoids and two secoirridoid glycosides, identified as kaempferol-3-O-neohesperoside (1), rutin (2), oleuropein (3), and ligstroside (4).
Among the isolated compounds from most active fraction (BuOH-F), rutin (2) displayed the highest anthelmintic activity in a dose-dependent activity with IC of 41.04 μg/mL against H. muscae adult worm, followed by ligstroside (4) with IC of 50.56 μg/mL.
These findings could advocate the traditional use of J. grandiflorum L. and provide further insight into the anthelmintic activity of flavonoids.
素馨花 L. 是一种药用植物，在传统医学中广泛用作驱虫药，用于治疗癣菌感染、溃疡、口腔炎、皮肤病和伤口。
据报道，不同寄生虫对目前使用的化学物质产生了耐药性。因此，越来越需要更有效和更安全的驱虫剂。因此，本研究旨在通过生物测定指导的分离和具有驱虫活性的活性成分的分离，验证其作为驱虫剂的传统用途，对素馨花亚种的甲醇提取物进行研究。 Floribundum (JGTE)。
将 JGTE 分为二氯甲烷 (DCM-F) 和正丁醇 (BuOH-F) 馏分。用两种线虫；一种绦虫幼虫阶段和一种节肢动物，在体外对 JGTE、馏分和分离的化合物进行驱虫活性测试。从最活跃的馏分 (BuOH-F) 中分离出四种主要化合物，包括两种类黄酮和两种裂环环烯醚萜糖苷，鉴定为山奈酚-3-O-新橙皮苷（1）、芦丁（2）、橄榄苦苷（3）和 Ligstroside (4)。
从最活跃的馏分 (BuOH-F) 中分离出的化合物中，芦丁（2）对 H. muscae 成虫的驱虫活性最高，呈剂量依赖性，IC 为 41.04μg/mL，其次是 Ligstroside (4)，IC 为 50.56μg/mL。
这些发现可以支持素馨花的传统用途，并为类黄酮的驱虫活性提供进一步的了解。
