Institute of Chemistry, Saint Petersburg State University, Universitetskii Prospect 26, Saint Petersburg, 198504, Russia.
Pavlov First Saint Petersburg State Medical University, L'va Tolstogo Ulitsa 6-8, Saint Petersburg, 197022, Russia; Almazov National Medical Research Centre, Akkuratova Ulitsa 2, Saint Petersburg, 197341, Russia.
Colloids Surf B Biointerfaces. 2020 Dec;196:111338. doi: 10.1016/j.colsurfb.2020.111338. Epub 2020 Aug 27.
Amino acid adducts of light fullerenes have a potential of application in a variety of fields of biomedicine, that is reactive oxygen species scavenging activity, anticancer activity, viruses and bacteria inactivation etc. In this work, the water-soluble C fullerene derivative with l-hydroxyproline (C(CHNO), C-Hyp) was studied. Extensive biomedical investigation of this compound, namely, antiradical activity in the reaction with stable diphenylpicrylhydrazyl radical, the binding to human serum albumin, photodynamic properties, cytotoxicity in glioblastoma A172 and lung carcinoma A549 cell lines, erythrocytes haemolysis, platelet aggregation, genotoxicity on human peripheral blood mononuclear cells was conducted. Moreover, the dynamic and structural characteristics of C-Hyp-HO binary system were obtained using molecular dynamic (MD) method, and size distribution along with ζ-potentials of C-Hyp associates was measured.
富勒烯氨基酸加合物在生物医学的多个领域具有应用潜力，例如清除活性氧物种、抗癌活性、病毒和细菌失活等。在这项工作中，研究了具有 l-羟脯氨酸的水溶性 C fullerene 衍生物（C(CHNO)，C-Hyp）。对该化合物进行了广泛的生物医学研究，即与稳定的二苯基苦基肼自由基反应的抗自由基活性、与人血清白蛋白的结合、光动力特性、在神经胶质瘤 A172 和肺癌 A549 细胞系中的细胞毒性、红细胞溶血、血小板聚集、对人外周血单个核细胞的遗传毒性。此外，还使用分子动力学 (MD) 方法获得了 C-Hyp-HO 二元体系的动态和结构特征，并测量了 C-Hyp 配合物的尺寸分布和 ζ-电势。
