Pavlov First Saint Petersburg State Medical University, 6-8 L'va Tolstogo ulitsa, Saint Petersburg, 197022, Russia; Institute of Chemistry, Saint Petersburg State University, 26 Universitetskii prospekt, Saint Petersburg, 198504, Russia; A. M. Granov Russian Research Centre for Radiology and Surgical Technologies, 70 Leningradskaya Ulitsa, Saint Petersburg, 197758, Russia.
Pavlov First Saint Petersburg State Medical University, 6-8 L'va Tolstogo ulitsa, Saint Petersburg, 197022, Russia; Almazov National Medical Research Centre, 2 Akkuratova ulitsa, Saint Petersburg, 197341, Russia.
J Biotechnol. 2021 Apr 10;331:83-98. doi: 10.1016/j.jbiotec.2021.03.007. Epub 2021 Mar 13.
Silica is silicon dioxide, which, depending on the production method, can exist in various amorphous forms with varying specific surface area, particle size, pore volume and size, and, as a result, with different physicochemical and sorption characteristics. The presence of silanol groups on the surface of silicas provides the possibility of its further functionalisation. In addition, the developed specific surface of Aerosil allows to obtain composites with a high content of biologically active substances. In this work, we studied the biocompatibility of a composite based on Aerosil 380 and carboxylated fullerene C[C(COOH)], namely: haemolysis (spontaneous and photoinduced), platelet aggregation, binding to HSA, cyto- and genotoxicity, antiradical activity. Interest in the creation of this nanomaterial is due to the fact that carboxylated fullerenes have potential applications in various fields of biomedicine, including the ability to bind reactive oxygen species, inhibition of tumour development, inactivation of viruses and bacteria. The obtained composite can be used for the immobilisation of various drugs and the further development of drugs for theranostics.
硅石是二氧化硅，根据生产方法的不同，它可以以各种无定形形式存在，具有不同的比表面积、粒径、孔体积和大小，因此具有不同的物理化学和吸附特性。硅石表面的硅醇基团的存在提供了进一步官能化的可能性。此外，Aerosil 开发的高比表面积允许获得高含量生物活性物质的复合材料。在这项工作中，我们研究了基于 Aerosil 380 和羧基化富勒烯 C[C(COOH)]的复合材料的生物相容性，即：溶血（自发和光诱导）、血小板聚集、与 HSA 的结合、细胞毒性和遗传毒性、抗自由基活性。对这种纳米材料的研究兴趣源于羧基化富勒烯在生物医学的各个领域的潜在应用，包括结合活性氧、抑制肿瘤发展、灭活病毒和细菌的能力。所得到的复合材料可用于固定各种药物，并进一步开发用于治疗和诊断的药物。
