Tipping P G, Buchanan R R, Riglar A G, Dimech W J, Littlejohn G O, Holdsworth S R
Department of Medicine, Monash University, Prince Henry's Hospital, Melbourne, Australia.
Br J Rheumatol. 1988 Jun;27(3):206-10. doi: 10.1093/rheumatology/27.3.206.
Four commercially available assays were compared with a 14C DNA Farr assay for their ability to detect anti-DNA antibodies in 119 sera from 109 patients with systemic lupus erythematosus (SLE) and 25 control sera. The 14C DNA Farr assay was the most sensitive and specific assay (SLE, 57% positive, controls, 0%). A commercial 125I DNA Farr assay was significantly less sensitive (SLE, 39% positive). The fluorescence human chromosomal preparation assay was as sensitive as the 14C DNA Farr assay (SLE, 58% positive) but less specific (controls, 8% positive). The immunofluorescence Crithidia luciliae assay was specific, but less sensitive (SLE, 37% positive) than the 14C DNA Farr assay. Adaptation of Crithidia to immunoperoxidase did not alter its sensitivity or performance. These results confirm that the 14C DNA Farr assay, locally refined and performed by experienced hands, is the most sensitive and specific assay for anti-DNA antibodies. The 125I DNA Farr was no more sensitive than the Crithidia assay but considerably more laborious. The human chromosomal preparation may be suitable as a rapid screening test for anti-DNA antibodies.
将四种市售检测方法与14C DNA Farr检测法进行比较，以评估它们检测109例系统性红斑狼疮（SLE）患者的119份血清及25份对照血清中抗DNA抗体的能力。14C DNA Farr检测法是最敏感和特异的检测方法（SLE患者中阳性率为57%，对照血清中为0%）。一种市售的125I DNA Farr检测法敏感性显著较低（SLE患者中阳性率为39%）。荧光人染色体制备检测法的敏感性与14C DNA Farr检测法相同（SLE患者中阳性率为58%），但特异性较低（对照血清中阳性率为8%）。免疫荧光利什曼原虫检测法具有特异性，但敏感性低于14C DNA Farr检测法（SLE患者中阳性率为37%）。将利什曼原虫检测法改编为免疫过氧化物酶检测法并未改变其敏感性或检测性能。这些结果证实，经本地改进并由经验丰富的人员操作的14C DNA Farr检测法是检测抗DNA抗体最敏感和特异的检测方法。125I DNA Farr检测法的敏感性并不高于利什曼原虫检测法，但操作要繁琐得多。人染色体制备检测法可能适合作为抗DNA抗体的快速筛查试验。
