Jansen E M, Deng J S, Beutner E H, Kumar V, Chorzelski T P
Am J Clin Pathol. 1987 Apr;87(4):461-9. doi: 10.1093/ajcp/87.4.461.
The reactions of sera from 15 selected patients with connective tissue diseases and 4 selected control people were compared with the use of five commercial kits detecting anti-nDNA antibodies by indirect immunofluorescence on Crithidia luciliae. The cases with systemic lupus erythematosus (SLE) and a related condition reacted with the kinetoplasts of the C. luciliae in each kit tested with one exception, notably a case of drug-induced LE. The four control cases selected for a trace of staining of the nuclei of C. luciliae gave negative reactions with the kinetoplasts. The titer for each individual positive serum varied from 1.36 to 2.67 (mean SG 2.04) geometric standard deviation units. The staining pattern of sera positive for anti-nDNA antibodies on C. luciliae included reactivity of the kinetoplast with or without nuclear staining. The drug-induced LE serum produced only nuclear staining with no significant kinetoplast staining, i.e., a negative test for anti-nDNA antibodies. Patient control sera stained only the nucleus when any reactivity on C. luciliae was present. Generally, there were no major differences in titers and patterns of sera when comparisons were made between manufacturers. The sera were also tested by the Farr radioimmunoassay and the latex nucleoprotein test. The results of both of these assays correlated in most cases with the C. luciliae reactions.
使用五种通过间接免疫荧光法在利什曼原虫上检测抗nDNA抗体的商业试剂盒，比较了15例选定的结缔组织病患者和4例选定的对照者血清的反应。除1例药物性狼疮外，系统性红斑狼疮（SLE）及相关病症的病例在每个测试试剂盒中均与利什曼原虫的动基体发生反应。为利什曼原虫细胞核微量染色而选择的4例对照病例，其动基体反应呈阴性。每个阳性血清个体的滴度在1.36至2.67（几何标准差单位平均为2.04）之间。抗nDNA抗体阳性血清在利什曼原虫上的染色模式包括动基体反应（有或无核染色）。药物性狼疮血清仅产生核染色，动基体无明显染色，即抗nDNA抗体检测呈阴性。当利什曼原虫出现任何反应性时，患者对照血清仅对细胞核染色。一般来说，不同厂家试剂盒之间血清的滴度和模式没有重大差异。这些血清还通过Farr放射免疫测定法和乳胶核蛋白试验进行了检测。在大多数情况下，这两种检测方法的结果与利什曼原虫反应相关。
