Sarvas H, Gripenberg M, Leirisalo-Repo M
Acta Pathol Microbiol Immunol Scand C. 1985 Feb;93(1):13-8. doi: 10.1111/j.1699-0463.1985.tb02915.x.
Sixty-six sera from 23 patients with systemic lupus erythematosus (SLE), 26 sera from patients with rheumatoid arthritis (RA), and 22 sera from normal healthy subjects were tested for the presence of antibodies against native (ds) DNA by the Crithidia luciliae immunofluorescence test and by the Farr assay, and for the presence of antibodies against denaturated (ss) DNA by the enzyme-linked immunosorbent assay (ELISA). Anti-dsDNA antibodies were detected in 57% of the SLE patients by the Crithidia test and in 65% by the Farr assay. Two of the RA sera were positive in the Crithidia test, whereas all were Farr negative. Anti-ssDNA antibodies of IgG class could be detected in 74% of the SLE patients and in none of the RA sera, while anti-ssDNA antibodies of IgM class were found in 26% of the SLE patients and in one RA serum. There was a good correlation between the results of the Farr assay and the IgG-anti-ssDNA ELISA but no agreement was found between the results of the Farr assay and the Crithidia test. We also measured the amount of C-reactive protein (CRP) in the sera but no correlation was seen between the levels of CRP and anti-DNA antibodies. We conclude that the demonstration of anti-ssDNA antibodies of IgG class is a good screening method in the diagnosis of SLE, and that antibodies against native DNA should be determined, preferably both by the Crithidia test and the Farr assay to confirm the diagnosis and in the follow-up of the patients.
采用利什曼原虫免疫荧光试验和法尔测定法检测了23例系统性红斑狼疮（SLE）患者的66份血清、类风湿性关节炎（RA）患者的26份血清以及正常健康受试者的22份血清中抗天然（双链）DNA抗体的存在情况，并采用酶联免疫吸附测定法（ELISA）检测了抗变性（单链）DNA抗体的存在情况。通过利什曼原虫试验，57%的SLE患者检测到抗双链DNA抗体，通过法尔测定法这一比例为65%。2份RA血清在利什曼原虫试验中呈阳性，而在法尔测定中均为阴性。74%的SLE患者可检测到IgG类抗单链DNA抗体，RA血清中均未检测到，而26%的SLE患者和1份RA血清中发现有IgM类抗单链DNA抗体。法尔测定结果与IgG抗单链DNA ELISA结果之间存在良好相关性，但法尔测定结果与利什曼原虫试验结果之间未发现一致性。我们还测量了血清中C反应蛋白（CRP）的含量，但未发现CRP水平与抗DNA抗体之间存在相关性。我们得出结论，IgG类抗单链DNA抗体的检测是SLE诊断的一种良好筛查方法，并且应测定抗天然DNA抗体，最好同时采用利什曼原虫试验和法尔测定法来确诊并对患者进行随访。
