Kim Bohyun, Seo Hyemi, Lee Jin Hyung, Kim Sunghyun, Choi Won Il, Sung Daekyung, Moon Eunpyo
Department of Biological Science, College of Natural Sciences, Ajou University, 206, World cup-ro, Yeongtong-gu, Suwon, Gyeonggi-do 16499, Korea.
Center for Convergence Bioceramic Materials, Korea Institute of Ceramic Engineering and Technology, 202, Osongsaengmyeong 1-ro, Osong-eup, Heungdeok-gu, Cheongju, Chungbuk 28160, Korea.
Polymers (Basel). 2020 Sep 1;12(9):1992. doi: 10.3390/polym12091992.
Suppression of pathogenic bacterial growth to increase food and agricultural productivity is important. We previously developed novel hexapeptides (KCM12 and KCM21) with antimicrobial activities against various phytopathogenic bacteria and N plasma-treated buffer (NPB) as an alternative method for bacterial inactivation and as an antibiofilm agent of crops. Here, we developed an enhanced antibiofilm method based on antimicrobial hexapeptides with N plasma-treated buffer against plant pathogens. Our results demonstrated that hexapeptides effectively inhibited the growth of pv. DC3000 ( DC3000) and the biofilm it formed. Potent biofilm formation-inhibiting effects of hexapeptides were observed at concentrations of above 20 µM, and samples treated with hexapeptide above 100 µM reduced the ability of the bacteria to produce biofilm by 80%. 3D confocal laser scanning microscopy imaging data revealed that the antimicrobial activity of hexapeptides was enough to affect the cells embedded inside the biofilm. Finally, combination treatment with NPB and antimicrobial hexapeptides increased the antibiofilm effect compared with the effect of single processing against multilayered plant pathogen biofilms. These findings show that the combination of hexapeptides and NPB can be potentially applied for improving crop production.
抑制致病细菌生长以提高粮食和农业生产力至关重要。我们之前开发了具有抗多种植物致病细菌活性的新型六肽（KCM12和KCM21）以及经氮等离子体处理的缓冲液（NPB），将其作为细菌灭活的替代方法和作物的抗生物膜剂。在此，我们基于具有抗微生物活性的六肽与经氮等离子体处理的缓冲液，开发了一种增强的针对植物病原体的抗生物膜方法。我们的结果表明，六肽能有效抑制丁香假单胞菌番茄致病变种（Pseudomonas syringae pv. tomato DC3000，DC3000）的生长及其形成的生物膜。在浓度高于20 μM时观察到六肽对生物膜形成有显著的抑制作用，用浓度高于100 μM的六肽处理的样品使细菌产生生物膜的能力降低了80%。三维共聚焦激光扫描显微镜成像数据显示，六肽的抗菌活性足以影响生物膜内包埋的细胞。最后，与针对多层植物病原体生物膜的单一处理效果相比，NPB与抗菌六肽的联合处理增强了抗生物膜效果。这些发现表明，六肽与NPB的组合可能适用于提高作物产量。
