Jee Seung-Cheol, Kim Min, Sung Jung-Suk, Kadam Avinash A
Department of Life Science, College of Life Science and Biotechnology, Dongguk University-Seoul, Biomedi Campus, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, Korea.
Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Biomedi Campus, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, Gyeonggi-do, Korea.
Polymers (Basel). 2020 Dec 17;12(12):3032. doi: 10.3390/polym12123032.
Removal of biofilms is extremely pivotal in environmental and medicinal fields. Therefore, reporting the new-enzymes and their combinations for dispersal of infectious biofilms can be extremely critical. Herein, for the first time, we accessed the enzyme "protease from bovine pancreas type-I (PtI)" for anti-biofilm properties. We further investigated the anti-biofilm potential of PtI in combination with α-amylase from . (αA). PtI showed a very significant biofilm inhibition effect (86.5%, 88.4%, and 67%) and biofilm prevention effect (66%, 64%, and 70%), against the , , and MRSA, respectively. However, the new enzyme combination (-PtI+αA) exhibited biofilm inhibition effect (78%, 90%, and 93%) and a biofilm prevention effect (44%, 51%, and 77%) against , , and MRSA, respectively. The studied enzymes were found not to be anti-bacterial against the , , and MRSA. In summary, the PtI exhibited significant anti-biofilm effects against , MRSA, and . -PtI+αA exhibited enhancement of the anti-biofilm effects against and MRSA biofilms. Therefore, this study revealed that this -PtI+αA enzymatic system can be extremely vital for the treatment of biofilm complications resulting from , , and MRSA.
生物膜的清除在环境和医学领域极为关键。因此，报道用于驱散感染性生物膜的新酶及其组合可能极其重要。在此，我们首次研究了“牛胰I型蛋白酶（PtI）”的抗生物膜特性。我们进一步研究了PtI与来自[具体来源未给出]的α-淀粉酶（αA）联合使用时的抗生物膜潜力。PtI对[具体细菌未给出]、[具体细菌未给出]和耐甲氧西林金黄色葡萄球菌（MRSA）分别显示出非常显著的生物膜抑制效果（86.5%、88.4%和67%）和生物膜预防效果（66%、64%和70%）。然而，新的酶组合（-PtI + αA）对[具体细菌未给出]、[具体细菌未给出]和MRSA的生物膜抑制效果分别为（78%、90%和93%），生物膜预防效果分别为（44%、51%和77%）。研究发现这些酶对[具体细菌未给出]、[具体细菌未给出]和MRSA没有抗菌作用。总之，PtI对[具体细菌未给出]、MRSA和[具体细菌未给出]表现出显著的抗生物膜效果。-PtI + αA对[具体细菌未给出]和MRSA生物膜的抗生物膜效果有所增强。因此，本研究表明这种-PtI + αA酶系统对于治疗由[具体细菌未给出]、[具体细菌未给出]和MRSA引起的生物膜并发症可能极其重要。
Zotero 插件
