Restrepo-Osorio Catalina, Gil-Correal Alejandro, Chamorro-Gutiérrez Lina, Ramírez-Ríos Viviana, Álvarez Javier C, Villanueva-Mejía Diego
Department of Biological Sciences, CIBIOP Research Group, Universidad EAFIT, Medellín, Colombia.
BMC Res Notes. 2020 Sep 3;13(1):414. doi: 10.1186/s13104-020-05257-1.
It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi (Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot development for this species.
Shoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species.
有必要改进基于体外细胞组织培养的生物技术平台，以支持美藤果（Plukenetia volubilis L.）研究项目，并利用其油籽中高含量多不饱和脂肪酸的营养价值。在此，我们开发了一种针对该物种的快速高效的诱导和直接体外芽发育方法。
从下胚轴外植体产生了芽。在添加了1毫克/升噻苯隆和0.5毫克/升玉米素，并补充了L-谷氨酰胺、腺嘌呤半硫酸盐和L-精氨酸的木本植物培养基中获得了最高的器官发生反应。使用该培养基获得的芽被转移并用不同浓度的吲哚-3-丁酸和1-萘乙酸进行继代培养以诱导生根。首次进行了组织学分析，支持该物种的直接器官发生发育。获得的小植株以80%的成活率转移到体外。通过随机扩增多态性DNA分析证实了再生植株的遗传稳定性。所有结果表明，有可能刺激下胚轴直接形成芽，以支持该物种的可持续利用。
