从中国渤海分离得到一株新型噬藻体 S-H68 及其基因组测序。

The isolation and genome sequencing of a novel cyanophage S-H68 from the Bohai Sea, China.

机构信息

College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.

College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China; Key Lab of Polar Oceanography and Global Ocean Change, Ocean University of China, Qingdao 266003, China.

出版信息

Mar Genomics. 2020 Oct;53:100739. doi: 10.1016/j.margen.2019.100739. Epub 2020 Jan 2.

DOI:10.1016/j.margen.2019.100739

PMID:32883437

Abstract

Cyanobacteria, also known as bule-green algae, are capable of photosynthesis and have a fixed carbon and nitrogen effect. The virus that specifically infects cyanobacteria is called the cyanophage. Cyanophages play a key role in building microbial communities. However, only a small number of cyanophages have been reported so far. In this study, a novel Synechococcus cyanophage S-H68 was isolated from the Bohai Sea of China. Transmission electron microscope observations showed that S-H68 has an icosahedral head, 66 ± 1 nm in diameter, and a tail with a length of 107 ± 1 nm, and should be grouped into the family Siphoviridae. To better understand the genetic diversity of this cyanophage, the complete genome was characterized. It consists of 79,639 -bp -length double-stranded DNA with a GC content of 59.8% and is predicted to have 117 open reading frames (ORFs) with an average length of 655 nucleotides. Using the BLASTN tool in the NCBI database for genome comparison, there was no significant similarity between S-H68 and other known cyanophages. So the present study added a new Siphoviridae cyanophage to the marine phage dataset.

摘要

蓝藻，亦称蓝绿藻，能够进行光合作用，具有固定碳氮的作用。专门感染蓝藻的病毒称为噬藻体。噬藻体在构建微生物群落方面发挥着关键作用。然而，迄今为止，仅报道了少数几种噬藻体。本研究从中国渤海分离到一株新型聚球藻噬藻体 S-H68。透射电镜观察显示，S-H68 具有二十面体头部，直径为 66±1nm，尾部长度为 107±1nm，应归属于肌尾噬菌体科。为了更好地了解该噬藻体的遗传多样性，对其全基因组进行了测序和分析。该噬藻体基因组为 79639bp 的双链 DNA，GC 含量为 59.8%，预测含有 117 个开放阅读框（ORFs），平均长度为 655 个核苷酸。使用 NCBI 数据库中的 BLASTN 工具进行基因组比较，发现 S-H68 与其他已知噬藻体之间没有显著的相似性。因此，本研究为海洋噬菌体数据集增加了一株新型肌尾噬菌体科噬藻体。