β-内酰胺酶产生的泌尿道致病性大肠埃希菌（UPEC）菌株在孟加拉国的基因型-表型相关性。
Genotype-phenotype correlation of β-lactamase-producing uropathogenic Escherichia coli (UPEC) strains from Bangladesh.
机构信息
NSU Genome Research Institute (NGRI), North South University, Dhaka, Bangladesh.
Department of Biochemistry and Microbiology, North South University, Dhaka, Bangladesh.
出版信息
Sci Rep. 2020 Sep 3;10(1):14549. doi: 10.1038/s41598-020-71213-5.
Escherichia coli is a pathogen commonly encountered in clinical laboratories, and is capable of causing a variety of diseases, both within the intestinal tract (intestinal pathogenic strains) and outside (extraintestinal pathogenic E. coli, or ExPEC). It is associated with urinary tract infections (UTIs), one of the most common infectious diseases in the world. This report represents the first comparative analysis of the draft genome sequences of 11 uropathogenic E. coli (UPEC) strains isolated from two tertiary hospitals located in Dhaka and Sylhet, Bangladesh, and is focused on comparing their genomic characteristics to each other and to other available UPEC strains. Multilocus sequence typing (MLST) confirmed the strains belong to ST59, ST131, ST219, ST361, ST410, ST448 and ST4204, with one of the isolates classified as a previously undocumented ST. De novo identification of the antibiotic resistance genes bla, bla, bla and bla was determined, and phenotypic-genotypic analysis of virulence revealed significant heterogeneity within UPEC phylogroups.
大肠杆菌是临床实验室中常见的病原体，能够引起多种疾病，包括肠道内（肠道致病性菌株）和肠道外（肠外致病性大肠杆菌，或 ExPEC）。它与尿路感染（UTIs）有关，这是世界上最常见的传染病之一。本报告代表了对来自孟加拉国达卡和锡尔赫特的两家三级医院的 11 株尿路致病性大肠杆菌（UPEC）分离株的基因组草图序列的首次比较分析，重点是比较它们的基因组特征彼此之间以及与其他可用的 UPEC 菌株。多位点序列分型（MLST）证实这些菌株属于 ST59、ST131、ST219、ST361、ST410、ST448 和 ST4204，其中一个分离株被归类为以前未记录的 ST。确定了 bla、bla、bla 和 bla 等抗生素耐药基因的从头鉴定，以及毒力的表型-基因型分析显示 UPEC phylogroups 内存在显著的异质性。
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