Division of Neurosciences, Pablo de Olavide University, Seville 4103, Spain.
Institute of Biochemistry, Charité-Universitätsmedizin Berlin, Berlin 10117, Germany.
Cereb Cortex. 2021 Jan 1;31(1):281-300. doi: 10.1093/cercor/bhaa225.
It is assumed that the claustrum (CL) is involved in sensorimotor integration and cognitive processes. We recorded the firing activity of identified CL neurons during classical eyeblink conditioning in rabbits, using a delay paradigm in which a tone was presented as conditioned stimulus (CS), followed by a corneal air puff as unconditioned stimulus (US). Neurons were identified by their activation from motor (MC), cingulate (CC), and medial prefrontal (mPFC) cortices. CL neurons were rarely activated by single stimuli of any modality. In contrast, their firing was significantly modulated during the first sessions of paired CS/US presentations, but not in well-trained animals. Neuron firing rates did not correlate with the kinematics of conditioned responses (CRs). CL local field potentials (LFPs) changed their spectral power across learning and presented well-differentiated CL-mPFC/CL-MC network dynamics, as shown by crossfrequency spectral measurements. CL electrical stimulation did not evoke eyelid responses, even in trained animals. Silencing of synaptic transmission of CL neurons by the vINSIST method delayed the acquisition of CRs but did not affect their presentation rate. The CL plays an important role in the acquisition of associative learning, mostly in relation to the novelty of CS/US association, but not in the expression of CRs.
据假设，屏状核（CL）参与感觉运动整合和认知过程。我们使用延迟范式在兔子中记录了已识别的 CL 神经元在经典眨眼条件反射期间的放电活动，其中声音作为条件刺激（CS）呈现，随后是角膜空气喷射作为非条件刺激（US）。神经元通过它们来自运动（MC）、扣带（CC）和内侧前额叶（mPFC）皮质的激活来识别。CL 神经元很少被任何单一刺激模式激活。相比之下，它们的放电在 CS/US 配对呈现的前几个阶段明显受到调制，但在训练有素的动物中则没有。神经元的放电率与条件反应（CR）的运动学无关。CL 局部场电位（LFPs）在学习过程中改变了它们的频谱功率，并通过交叉频率谱测量显示了良好区分的 CL-mPFC/CL-MC 网络动力学。CL 电刺激甚至在训练有素的动物中也不会引起眼睑反应。使用 vINSIST 方法沉默 CL 神经元的突触传递会延迟 CR 的获得，但不会影响它们的呈现率。CL 在获得联想学习中起着重要作用，主要与 CS/US 关联的新颖性有关，但与 CR 的表达无关。
