Division of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, India.
Division of Physiology and Climatology, ICAR-Indian Veterinary Research Institute, Izatnagar, India.
Anim Biotechnol. 2022 Aug;33(4):629-637. doi: 10.1080/10495398.2020.1813151. Epub 2020 Sep 4.
Classical Swine Fever (CSF) is an extremely infectious and deadly disease of pigs and wild boars caused by the CSF virus (CSFV) which is a member of the Pestivirus genus and the family . This study was designed to detect the permissibility and replication of CSFV in mesenchymal stem cells (MSCs) monolayer derived from Porcine Wharton's jelly. Porcine Wharton's jelly MSCs (pWJ-MSCs) were expanded and propagated for more than 81 generations and third passage pWJ-MSCs were characterized as per standard criteria i.e., growth characteristics, trilineage differentiation potential and molecular characterization for pluripotency and stem cell surface markers. Porcine WJ tissue samples found negative for CSFV by RT-PCR test were processed further for the isolation of pWJ-MSCs and CSFV was propagated over the characterized pWJ-MSCs monolayer. No cytopathic effect was observed, which was consistent with non-cytopathic nature of CSFV. The replication of CSFV in pWJ-MSCs was affirmed by RT-PCR and demonstration of viral antigen in the cytoplasm of virus infected cells by immuno-staining technique. In total, three different CSFV isolates were propagated in pWJ-MSCs. Primary pWJ-MSCs permitted CSFV replication to good titer. To the best of our information, this is the first ever report of isolation of CSFV in pWJ-MSCs.
经典猪瘟（CSF）是一种极其具有传染性和致命性的猪和野猪疾病，由 CSF 病毒（CSFV）引起，CSFV 是瘟病毒属和 科的成员。本研究旨在检测 CSFV 在源自猪 Wharton 胶的间充质干细胞（MSCs）单层中的允许性和复制。猪 Wharton 胶 MSCs（pWJ-MSCs）被扩展和繁殖超过 81 代，并且第三代 pWJ-MSCs 根据标准标准进行特征化，即生长特征、三系分化潜能和多能性和干细胞表面标志物的分子特征。通过 RT-PCR 试验发现猪 WJ 组织样本对 CSFV 呈阴性，进一步处理用于分离 pWJ-MSCs，并在特征化的 pWJ-MSCs 单层上繁殖 CSFV。未观察到细胞病变效应，这与 CSFV 的非致细胞病变性质一致。通过 RT-PCR 和免疫染色技术在感染细胞的细胞质中显示病毒抗原证实了 CSFV 在 pWJ-MSCs 中的复制。总共在 pWJ-MSCs 中繁殖了三种不同的 CSFV 分离株。原代 pWJ-MSCs 允许 CSFV 复制到良好的效价。就我们所知，这是首次在 pWJ-MSCs 中分离 CSFV 的报道。
