• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

猪脂肪来源与华通氏胶来源间充质干细胞的比较分析

Comparative Analysis of Porcine Adipose- and Wharton's Jelly-Derived Mesenchymal Stem Cells.

作者信息

Kim Ga Yeon, Choi Gyu Tae, Park Jinryong, Lee Jeongeun, Do Jeong Tae

机构信息

Department of Stem Cell and Regenerative Biotechnology, KU Institute of Technology, Konkuk University, Seoul 05029, Republic of Korea.

3D Tissue Culture Research Center, Konkuk University, Seoul 05029, Republic of Korea.

出版信息

Animals (Basel). 2023 Sep 17;13(18):2947. doi: 10.3390/ani13182947.

DOI:10.3390/ani13182947
PMID:37760347
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10525484/
Abstract

Mesenchymal stem cells (MSCs) are promising candidates for tissue regeneration, cell therapy, and cultured meat research owing to their ability to differentiate into various lineages including adipocytes, chondrocytes, and osteocytes. As MSCs display different characteristics depending on the tissue of origin, the appropriate cells need to be selected according to the purpose of the research. However, little is known of the unique properties of MSCs in pigs. In this study, we compared two types of porcine mesenchymal stem cells (MSCs) isolated from the dorsal subcutaneous adipose tissue (adipose-derived stem cells (ADSCs)) and Wharton's jelly of the umbilical cord (Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs)) of 1-day-old piglets. The ADSCs displayed a higher proliferation rate and more efficient differentiation potential into adipogenic and chondrogenic lineages than that of WJ-MSCs; conversely, WJ-MSCs showed superior differentiation capacity towards osteogenic lineages. In early passages, ADSCs displayed higher proliferation rates and mitochondrial energy metabolism (measured based on the oxygen consumption rate) compared with that of WJ-MSCs, although these distinctions diminished in late passages. This study broadens our understanding of porcine MSCs and provides insights into their potential applications in animal clinics and cultured meat science.

摘要

间充质干细胞（MSCs）因其能够分化为包括脂肪细胞、软骨细胞和骨细胞在内的各种谱系，在组织再生、细胞治疗和培养肉研究方面具有广阔的应用前景。由于MSCs根据其来源组织表现出不同的特性，因此需要根据研究目的选择合适的细胞。然而，对于猪MSCs的独特特性知之甚少。在本研究中，我们比较了从1日龄仔猪的背部皮下脂肪组织（脂肪来源干细胞（ADSCs））和脐带华通氏胶（华通氏胶来源间充质干细胞（WJ-MSCs））中分离出的两种猪间充质干细胞。与WJ-MSCs相比，ADSCs表现出更高的增殖率以及向脂肪生成和软骨生成谱系的更高效分化潜力；相反，WJ-MSCs在向成骨谱系的分化能力方面表现更优。在早期传代中，与WJ-MSCs相比，ADSCs表现出更高的增殖率和线粒体能量代谢（基于耗氧率测量），尽管这些差异在后期传代中逐渐减小。本研究拓宽了我们对猪MSCs的理解，并为其在动物临床和培养肉科学中的潜在应用提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4be3/10525484/adba34f8771d/animals-13-02947-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4be3/10525484/14deb600dad1/animals-13-02947-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4be3/10525484/a47eeb32d05d/animals-13-02947-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4be3/10525484/7f6902a819e9/animals-13-02947-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4be3/10525484/adba34f8771d/animals-13-02947-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4be3/10525484/14deb600dad1/animals-13-02947-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4be3/10525484/a47eeb32d05d/animals-13-02947-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4be3/10525484/7f6902a819e9/animals-13-02947-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4be3/10525484/adba34f8771d/animals-13-02947-g004.jpg

相似文献

1
Comparative Analysis of Porcine Adipose- and Wharton's Jelly-Derived Mesenchymal Stem Cells.猪脂肪来源与华通氏胶来源间充质干细胞的比较分析
Animals (Basel). 2023 Sep 17;13(18):2947. doi: 10.3390/ani13182947.
2
Osteogenic differentiation of human mesenchymal stem cells from adipose tissue and Wharton's jelly of the umbilical cord.来自脂肪组织和脐带华通氏胶的人间充质干细胞的成骨分化
Acta Biochim Pol. 2017;64(2):365-369. doi: 10.18388/abp.2016_1488. Epub 2017 Jun 10.
3
The effect of fibroblast growth factor on distinct differentiation potential of cord blood-derived unrestricted somatic stem cells and Wharton's jelly-derived mesenchymal stem/stromal cells.成纤维细胞生长因子对脐血来源的无限制体细胞干细胞和华通氏胶来源的间充质干/基质细胞不同分化潜能的影响。
Cytotherapy. 2015 Dec;17(12):1723-31. doi: 10.1016/j.jcyt.2015.09.007.
4
Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton's jelly and bone marrow-derived mesenchymal stem cells.细胞周期和WNT信号通路相关基因的差异表达导致了脐带来源间充质干细胞与骨髓来源间充质干细胞在生长和分化潜能上的差异。
Stem Cell Res Ther. 2017 Apr 26;8(1):102. doi: 10.1186/s13287-017-0555-9.
5
Expression Profile of New Marker Genes Involved in Differentiation of Human Wharton's Jelly-Derived Mesenchymal Stem Cells into Chondrocytes, Osteoblasts, Adipocytes and Neural-like Cells.涉及人脐带来源间充质干细胞向软骨细胞、成骨细胞、脂肪细胞和神经样细胞分化的新标记基因的表达谱。
Int J Mol Sci. 2023 Aug 18;24(16):12939. doi: 10.3390/ijms241612939.
6
Isolation and Characterization of Feline Wharton's Jelly-Derived Mesenchymal Stem Cells.猫源华通氏胶间充质干细胞的分离与鉴定
Vet Sci. 2021 Feb 7;8(2):24. doi: 10.3390/vetsci8020024.
7
Comparative analysis of mesenchymal stromal cells derived from rabbit bone marrow and Wharton's jelly for adipose tissue engineering.兔骨髓间充质干细胞与牙髓间充质干细胞用于脂肪组织工程的比较分析。
Connect Tissue Res. 2020 Nov;61(6):537-545. doi: 10.1080/03008207.2019.1631297. Epub 2019 Jun 18.
8
Characteristics and clinical applications of Wharton's jelly-derived mesenchymal stromal cells.牙髓基质细胞的特征和临床应用。
Curr Res Transl Med. 2020 Jan;68(1):5-16. doi: 10.1016/j.retram.2019.09.001. Epub 2019 Sep 19.
9
Isolation method and xeno-free culture conditions influence multipotent differentiation capacity of human Wharton's jelly-derived mesenchymal stem cells.分离方法和无血清培养条件影响人脐带华通氏胶间充质干细胞的多能分化能力。
Stem Cell Res Ther. 2013 Jul 11;4(4):81. doi: 10.1186/scrt232.
10
Isolation and characterization of Wharton's jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system.从牛脐带中分离和鉴定 Wharton's 胶源多能间充质基质细胞，并在无血清三维体系中维持。
BMC Biotechnol. 2012 May 4;12:18. doi: 10.1186/1472-6750-12-18.

引用本文的文献

1
Enhancing adipogenesis in Wharton's jelly multipotent mesenchymal stromal cells through lipidomic insights and fatty acid supplementation.通过脂质组学见解和脂肪酸补充增强脐带来源的多能间充质基质细胞的脂肪生成
Sci Rep. 2025 Aug 22;15(1):30962. doi: 10.1038/s41598-025-16867-9.
2
Evaluation of enzymatic protocols to optimize efficiency of bovine adipose tissue-derived mesenchymal stromal cell isolation.评估酶促方案以优化牛脂肪组织来源间充质基质细胞分离的效率。
NPJ Sci Food. 2024 Oct 1;8(1):70. doi: 10.1038/s41538-024-00313-7.
3
Influence of Gelatin on Adhesion, Proliferation, and Adipogenic Differentiation of Adipose Tissue-Derived Stem Cells Cultured on Soy Protein-Agarose Scaffolds.

本文引用的文献

1
Superior therapeutic activity of TGF-β-induced extracellular vesicles against interstitial cystitis.TGF-β 诱导的细胞外囊泡对间质性膀胱炎的治疗活性更高。
J Control Release. 2022 Aug;348:924-937. doi: 10.1016/j.jconrel.2022.06.045. Epub 2022 Jun 29.
2
Dysfunction of metabolic activity of bone marrow mesenchymal stem cells in aged mice.衰老小鼠骨髓间充质干细胞代谢活性障碍。
Cell Prolif. 2022 Mar;55(3):e13191. doi: 10.1111/cpr.13191. Epub 2022 Jan 27.
3
Mesenchymal stem cell treatment improves outcome of COVID-19 patients via multiple immunomodulatory mechanisms.
明胶对培养于大豆蛋白-琼脂糖支架上的脂肪组织来源干细胞的黏附、增殖及成脂分化的影响
Foods. 2024 Jul 17;13(14):2247. doi: 10.3390/foods13142247.
4
Cartilage-Specific Gene Expression and Extracellular Matrix Deposition in the Course of Mesenchymal Stromal Cell Chondrogenic Differentiation in 3D Spheroid Culture.在 3D 球体培养中骨髓基质细胞软骨分化过程中软骨特异性基因表达和细胞外基质沉积。
Int J Mol Sci. 2024 May 23;25(11):5695. doi: 10.3390/ijms25115695.
间充质干细胞治疗通过多种免疫调节机制改善 COVID-19 患者的预后。
Cell Res. 2021 Dec;31(12):1244-1262. doi: 10.1038/s41422-021-00573-y. Epub 2021 Oct 26.
4
Effects of fatty acids on T cell function: role in atherosclerosis.脂肪酸对 T 细胞功能的影响：在动脉粥样硬化中的作用。
Nat Rev Cardiol. 2021 Dec;18(12):824-837. doi: 10.1038/s41569-021-00582-9. Epub 2021 Jul 12.
5
Age-Related Changes in Bone-Marrow Mesenchymal Stem Cells.骨髓间充质干细胞的年龄相关性变化。
Cells. 2021 May 21;10(6):1273. doi: 10.3390/cells10061273.
6
Current Issues and Technical Advances in Cultured Meat Production: A Review.cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review. cultured meat production: A Review.
Food Sci Anim Resour. 2021 May;41(3):355-372. doi: 10.5851/kosfa.2021.e14. Epub 2021 May 1.
7
The Crosstalk of Adipose-Derived Stem Cells (ADSC), Oxidative Stress, and Inflammation in Protective and Adaptive Responses.脂肪来源的干细胞（ADSC）、氧化应激和炎症在保护和适应性反应中的串扰。
Int J Mol Sci. 2020 Dec 4;21(23):9262. doi: 10.3390/ijms21239262.
8
FABP3-mediated membrane lipid saturation alters fluidity and induces ER stress in skeletal muscle with aging.FABP3 介导的膜脂质饱和改变了衰老骨骼肌的流动性并诱导内质网应激。
Nat Commun. 2020 Nov 9;11(1):5661. doi: 10.1038/s41467-020-19501-6.
9
Isolation and propagation of classical swine fever virus in porcine Wharton's Jelly mesenchymal stem cells.猪源华通氏胶间充质干细胞中猪瘟病毒的分离与增殖。
Anim Biotechnol. 2022 Aug;33(4):629-637. doi: 10.1080/10495398.2020.1813151. Epub 2020 Sep 4.
10
Phenotype Characteristics and Osteogenic Differentiation Potential of Human Mesenchymal Stem Cells Derived from Amnion Membrane (HAMSCs) and Umbilical Cord (HUC-MSCs).源自羊膜（HAMSCs）和脐带（HUC-MSCs）的人骨髓间充质干细胞的表型特征和成骨分化潜能
Acta Inform Med. 2019 Jun;27(2):72-77. doi: 10.5455/aim.2019.27.72-77.