白叶藤叶提取物对黑眉锦蛇蛇毒在白化鼠和牛血中的体外和体内中和活性。

In Vitro and In Vivo Neutralizing Activity of Uvaria chamae Leaves Fractions on the Venom of Naja nigricollis in Albino Rat and Bovine Blood.

机构信息

Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria.

Department of Pharmacology and Therapeutics, Ahmadu Bello University, Zaria, Nigeria.

出版信息

Recent Pat Biotechnol. 2020;14(4):295-311. doi: 10.2174/1872208314666200903152129.

DOI:10.2174/1872208314666200903152129

PMID:32885765

Abstract

BACKGROUND

Snakebite envenomation is a global priority ranked top among other neglected tropical diseases. There is a folkloric claim that Uvaria chamae is beneficial for the management of snakebite and wounds in African ethnobotanical surveys. Besides, there are many registered patents asserting the health benefits of U. chamae.

OBJECTIVE

This study aimed to investigate U. chamae's potentials and identify candidates for the development of tools for the treatment and management of N. nigricollis envenomation.

METHODS

Freshly collected U. chamae leaves were air-dried, powdered, and extracted in methanol. The median lethal dose of the extract was determined and further fractionated with n-hexane, n-butanol and ethyl acetate. Each fraction was tested for neutralizing effect against venom-induced haemolytic, fibrinolytic, hemorrhagic, and cytotoxic activities.

RESULTS

U. chamae fractions significantly (p<0.05) neutralized the haemolytic activity of N. nigricollis venom in n-butanol; 31.40%, n-hexane; 33%, aqueous residue; 39.60% and ethyl acetate; 40.70% at the concentration of 100mg/ml of each fraction against 10mg/ml of the snake venom when compared to the positive control. The fibrinolytic activity of N. nigricollis venom was significantly (p<0.05) neutralized in n-hexane at 73.88%, n-butanol; 72.22% and aqueous residue; 72.22% by the fractions of U. chamae. In addition, haemorrhagic activity of N. nigricollis venom was significantly (p<0.05) neutralized by U. chamae fractions at the concentrations of 100mg/ml, 200mg/ml and 400mg/ml except for n-butanol and aqueous residues at 400 mg/ml.

CONCLUSION

U. chamae leaves fractions possess a high level of protection against N. nigricollis venoms-induced lethality and thus validate the pharmacological rationale for its usage in the management of N. nigricollis envenomation.

摘要

背景

蛇伤是全球优先事项之一，在其他被忽视的热带病中排名最高。在非洲民族植物学调查中，有一种民间说法认为 Uvaria chamae 有益于治疗和处理蛇伤和伤口。此外，有许多已注册的专利声称 U. chamae 具有健康益处。

目的

本研究旨在研究 U. chamae 的潜力，并确定其开发用于治疗和管理 N. nigricollis 中毒的工具的候选者。

方法

新鲜采集的 U. chamae 叶片风干、粉碎后用甲醇提取。测定提取物的半数致死量，并用正己烷、正丁醇和乙酸乙酯进一步分段。测试每个馏分对蛇毒诱导的溶血、纤溶、出血和细胞毒性活性的中和作用。

结果

U. chamae 各馏分显著（p<0.05）中和了 N. nigricollis 毒液在正丁醇中的溶血活性；31.40%，正己烷；33%，水残留物；39.60%和乙酸乙酯；40.70%，与 10mg/ml 蛇毒相比，各馏分在 100mg/ml 浓度下的阳性对照。N. nigricollis 毒液的纤溶活性在正己烷中被显著（p<0.05）中和，在正丁醇中为 73.88%，在正己烷中为 72.22%，在水残留物中为 72.22%，由 U. chamae 各馏分组成。此外，N. nigricollis 毒液的出血活性在 100mg/ml、200mg/ml 和 400mg/ml 浓度下被 U. chamae 馏分显著（p<0.05）中和，除了正丁醇和水残留物在 400mg/ml 浓度下。