Department of Physiology and Behavior, Universidade Federal do Rio Grande do Norte, Rio Grande do Norte, Brazil.
Alcohol Alcohol. 2020 Oct 20;55(6):581-590. doi: 10.1093/alcalc/agaa087.
Fetal alcohol spectrum disorder (FASD) is an umbrella term to describe the effects of ethanol (Eth) exposure during embryonic development, including several conditions from malformation to cognitive deficits. Zebrafish (Danio rerio) are a translational model popularly applied in brain disorders and drug screening studies due to its genetic and physiology homology to humans added to its transparent eggs and fast development. In this study, we investigated how early ethanol exposure affects zebrafish behavior during the initial growth phase.
Fish eggs were exposed to 0.0 (control), 0.25 and 0.5% ethanol at 24 h post-fertilization. Later, fry zebrafish (10 days old) were tested in a novel tank task and an inhibitory avoidance protocol to inquire about morphology and behavioral alterations.
Analysis of variance showed that ethanol doses of 0.25 and 0.5% do not cause morphological malformations and did not impair associative learning but increased anxiety-like behavior responses and lower exploratory behavior when compared to the control.
Our results demonstrate that one can detect behavioral abnormalities in the zebrafish induced by embryonic ethanol as early as 10 days post-fertilization and that alcohol increases anxious behavior during young development in zebrafish.
胎儿酒精谱系障碍（FASD）是一个伞式术语，用于描述胚胎发育过程中乙醇（Eth）暴露的影响，包括畸形到认知缺陷等几种情况。斑马鱼（Danio rerio）是一种转化模型，由于其与人类在遗传和生理上的同源性，以及其透明的卵子和快速的发育，在脑疾病和药物筛选研究中得到了广泛的应用。在这项研究中，我们研究了早期乙醇暴露如何影响斑马鱼在初始生长阶段的行为。
在受精后 24 小时，将鱼卵暴露于 0.0（对照）、0.25 和 0.5%的乙醇中。然后，将 10 天大的幼鱼在新鱼缸任务和抑制回避协议中进行测试，以探究形态和行为变化。
方差分析表明，0.25%和 0.5%的乙醇剂量不会导致形态畸形，也不会损害联想学习，但与对照组相比，会增加焦虑样行为反应和降低探索行为。
我们的结果表明，人们可以在受精后 10 天左右检测到胚胎乙醇引起的斑马鱼行为异常，并且酒精会增加幼鱼发育过程中的焦虑行为。
