State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Foods, School of Food Science Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, National Engineering Research Center for Functional Foods, School of Food Science Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Talanta. 2020 Nov 1;219:121343. doi: 10.1016/j.talanta.2020.121343. Epub 2020 Jul 7.
N-acylated homoserine lactones (AHLs), a class of auto-inducers produced by Gram-negative bacteria, are typical signaling molecules in quorum sensing (QS) systems. Importantly, AHLs play a key role in determining the virulence of foodborne pathogens and reflect the activity of spoilage bacteria. In this study, an eco-friendly fluorescence-sensing platform for the rapid and sensitive detection of AHLs was developed and characterized. Molecularly imprinted polymers embedded with yellow-emitting carbon quantum dots (CQDs) were obtained via the sol-gel process using furanone as an alternative template molecule, and long-wave-emitting CQDs with excellent optical properties were used as signal conversion materials. After template elution, the blotting cavities on the surface of the CQD@MIPs (molecularly imprinted polymers) were able to selectively recognize AHLs, demonstrating a stronger fluorescence response compared with the corresponding CQD@NIPs (non-imprinted polymers). Under optimal test conditions, a good linear relationship between the concentration of analyte and the relative fluorescence intensity of the CQD@MIPs was observed. The linear detection range was 0-2.0 μM, and the limit of detection (LOD) was 0.067 μM. Importantly, the proposed sensing platform functioned as an optical detection strategy that responded quickly (2 min) to AHLs. Additionally, this sensing platform was applied to the analysis of AHLs in bacterial supernatant samples with satisfactory results. More interestingly, the 3D-printing CQD@MIPs were tentative explored in this work, which was personalized and portable, has an advantage of point of care testing (POCT) detection in the future. Based on these results, this detection strategy has demonstrated substantial potential for application in and the field of food safety.
N-酰化高丝氨酸内酯（AHLs）是革兰氏阴性细菌产生的一类自动诱导物，是群体感应（QS）系统中典型的信号分子。重要的是，AHLs 在确定食源性病原体的毒力方面起着关键作用，并反映了腐败细菌的活性。在这项研究中，开发并表征了一种用于快速灵敏检测 AHLs 的环保荧光传感平台。通过溶胶-凝胶过程，使用呋喃酮作为替代模板分子，获得了嵌入黄色发射碳量子点（CQDs）的分子印迹聚合物，并且使用具有优异光学性能的长波长发射 CQDs 作为信号转换材料。模板洗脱后，CQD@MIPs（分子印迹聚合物）表面的印迹腔能够选择性地识别 AHLs，与相应的 CQD@NIPs（非印迹聚合物）相比，表现出更强的荧光响应。在最佳测试条件下，分析物浓度与 CQD@MIPs 的相对荧光强度之间观察到良好的线性关系。线性检测范围为 0-2.0 μM，检测限（LOD）为 0.067 μM。重要的是，所提出的传感平台作为光学检测策略，对 AHLs 的响应速度很快（2 分钟）。此外，该传感平台被应用于细菌上清液样品中 AHLs 的分析，结果令人满意。更有趣的是，在这项工作中，初步探索了 3D 打印的 CQD@MIPs，它具有个性化和便携性的特点，将来在即时检测（POCT）检测方面具有优势。基于这些结果，这种检测策略在食品安全领域具有很大的应用潜力。
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