State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, PR China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225127, PR China.
Biosens Bioelectron. 2016 Jan 15;75:411-9. doi: 10.1016/j.bios.2015.07.045. Epub 2015 Jul 21.
We have developed a novel and economical electrochemical sensor to measure Gram-negative bacterial quorum signaling molecules (AHLs) using magnetic nanoparticles and molecularly imprinted polymer (MIP) technology. Magnetic molecularly imprinted polymers (MMIPs) capable of selectively absorbing AHLs were successfully synthesized by surface polymerization. The particles were deposited onto a magnetic carbon paste electrode (MGCE) surface, and characterized by electrochemical measurements. Differential Pulse Voltammetry (DPV) was utilized to record the oxidative current signal that is characteristic of AHL. The detection limit of this assay was determined to be 8×10(-10)molL(-1) with a linear detection range of 2.5×10(-9)molL(-1) to 1.0×10(-7)molL(-1). This Fe3O4@SiO2-MIP-based electrochemical sensor is a valuable new tool that allows quantitative measurement of Gram-negative bacterial quorum signaling molecules. It has potential applications in the fields of clinical diagnosis or food analysis with real-time detection capability, high specificity, excellent reproducibility, and good stability.
我们开发了一种新颖且经济的电化学传感器，使用磁性纳米粒子和分子印迹聚合物 (MIP) 技术来测量革兰氏阴性细菌群体感应信号分子 (AHLs)。通过表面聚合成功合成了能够选择性吸收 AHL 的磁性分子印迹聚合物 (MMIP)。将颗粒沉积到磁性碳糊电极 (MGCE) 表面上，并通过电化学测量进行了表征。差分脉冲伏安法 (DPV) 用于记录 AHL 的特征氧化电流信号。该测定的检测限确定为 8×10(-10)molL(-1)，线性检测范围为 2.5×10(-9)molL(-1)至 1.0×10(-7)molL(-1)。这种基于 Fe3O4@SiO2-MIP 的电化学传感器是一种有价值的新工具，可用于定量测量革兰氏阴性细菌群体感应信号分子。它具有实时检测能力、高特异性、出色的重现性和良好的稳定性，因此在临床诊断或食品分析领域具有潜在的应用前景。
