Bio-Center, Gyeonggido Business and Science Accelerator, Gwanggyo-ro 147, Yeoungtong, Suwon, Gyeonggi 16229, Korea.
College of Pharmacy, Dankook University, Dandae-ro 119, Dongnam, Cheonan, Chungnam 31116, Korea.
Molecules. 2020 Sep 2;25(17):3997. doi: 10.3390/molecules25173997.
In the present study the effects and molecular mechanisms of wheat bran (WB), the hard outer layer of the wheat kernel used in food ingredients, on mast cell-mediated allergic responses in vitro and in vivo were investigated. The water extract of WB inhibited degranulation and expression of allergic and inflammatory mediators such as tumor necrosis factor-α, cyclooxygenase-2 and inducible nitric oxide synthase in antigen-stimulated RBL-2H3 cells. These anti-allergic activities of WB were mediated by the inactivation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase, which play important roles in degranulation and expression of various allergic and inflammatory molecules. In agreement with its in vitro effects, WB inhibited immunoglobulin E (IgE)/antigen-induced and compound 48/80-induced anaphylactic reactions in vivo. Taken together, these findings suggest the pharmacological potential of WB in the regulation of allergic diseases, including allergic rhinitis, atopic dermatitis, asthma and anaphylaxis.
在本研究中，研究了小麦麸（WB）（用于食品成分的小麦颗粒的硬外壳）对体外和体内肥大细胞介导的过敏反应的作用和分子机制。WB 的水提取物抑制了 RBL-2H3 细胞中抗原刺激引起的脱颗粒和过敏及炎症介质（如肿瘤坏死因子-α、环氧化酶-2 和诱导型一氧化氮合酶）的表达。WB 的这些抗过敏活性是通过细胞外信号调节激酶和 p38 丝裂原活化蛋白激酶的失活介导的，这两种激酶在脱颗粒和各种过敏及炎症分子的表达中发挥重要作用。与体外作用一致，WB 抑制了免疫球蛋白 E（IgE）/抗原诱导和化合物 48/80 诱导的过敏反应。总之，这些发现表明 WB 在调节过敏疾病（包括过敏性鼻炎、特应性皮炎、哮喘和过敏反应）方面具有药理学潜力。
