Department of Industrial Biotechnology, School of Engineering Science in Chemistry, Biotechnology and Health, Albanova University Centre, KTH Royal Institute of Technology, Roslagstullcbacken 21, Stockholm, Sweden; Water Research Laboratory, Riga Technical University, Kipsala, 6A-263, Riga, Latvia.
Water Research Laboratory, Riga Technical University, Kipsala, 6A-263, Riga, Latvia.
Microbiol Res. 2020 Dec;241:126586. doi: 10.1016/j.micres.2020.126586. Epub 2020 Aug 22.
Discharge of organic load from treated wastewater may cause environmental eutrophication. Recently, fungi have gained much attention due to their removal of pharmaceutical substances by enzymatic degradation and adsorption. However, the fungal effect in removing nutrients is less investigated. Therefore, two fungal species, the white-rot fungus T. versicolor as a laboratory strain and the mold A. luchuensis as an environmental isolate from the municipal wastewater treatment plant, were studied to determine the fungal potential for phosphorus, nitrogen, and the total organic carbon removal from municipal wastewater, carrying out a batch scale experiment to a fluidized bed pelleted bioreactor. During the batch scale experiment, the total removal (99.9 %) of phosphorus by T. versicolor was attained after a 6 h-long incubation period while the maximal removal efficiency (99.9 %) for phosphorus from A. luchuensis was gained after an incubation period of 24 h. Furthermore, both fungi showed that the pH adjustment to 5.5 kept the concentration of nitrogen constant and stabilized the total organic carbon reduction process for the entire incubation period. The results from the fluidized bed bioreactor demonstrated opposite tendencies on a nutrient removal comparing to a batch experiment where no significant effect on phosphorus, nitrogen, and total organics carbon reduction was observed. The obtained results from this study of batch and fluidized bed bioreactor experiments are a promising starting point for a successful fungal treatment optimization and application to wastewater treatment.
处理废水中有机负荷的排放可能会导致环境富营养化。最近，由于真菌通过酶降解和吸附去除药物物质，因此受到了广泛关注。然而，对于真菌去除营养物质的效果的研究较少。因此，研究了两种真菌，白腐真菌 T. versicolor 作为实验室菌株和来自城市污水处理厂的环境分离菌 A. luchuensis，以确定真菌从城市废水中去除磷、氮和总有机碳的潜力，在流化床颗粒生物反应器中进行了批量规模实验。在批量规模实验中，T. versicolor 在 6 小时的孵育期后实现了磷的总去除率（99.9%），而 A. luchuensis 从孵育期 24 小时后达到了最大的磷去除效率（99.9%）。此外，两种真菌都表明将 pH 值调节至 5.5 可以保持氮的浓度恒定，并稳定整个孵育期的总有机碳还原过程。流化床生物反应器的结果与批量实验相比表现出相反的趋势，对磷、氮和总有机物碳的去除没有明显影响。本研究中从批量和流化床生物反应器实验中获得的结果为真菌处理的成功优化和应用于废水处理提供了有希望的起点。
