重组人造血生长因子靶标的无血清培养分析：白细胞介素3和粒细胞/巨噬细胞集落刺激因子对早期发育阶段具有特异性。
Analysis in serum-free culture of the targets of recombinant human hemopoietic growth factors: interleukin 3 and granulocyte/macrophage-colony-stimulating factor are specific for early developmental stages.
作者信息
Sonoda Y, Yang Y C, Wong G G, Clark S C, Ogawa M
机构信息
Department of Medicine, Medical University of South Carolina, Charleston.
出版信息
Proc Natl Acad Sci U S A. 1988 Jun;85(12):4360-4. doi: 10.1073/pnas.85.12.4360.
We have used a serum-free culture system for enriched human hemopoietic progenitors to analyze the developmental stages and lineage specificities of the human hemopoietic colony-stimulating factors. None of the individual factors alone efficiently supported hemopoietic colony formation. Neither interleukin 3 nor granulocyte/macrophage-colony-stimulating factor alone or in combination effectively supported proliferation of progenitor cells. However, when combined with granulocyte-colony-stimulating factor or erythropoietin, these factors yielded neutrophil colonies or erythroid bursts, respectively. Serial observations of interleukin 3-supported cultures revealed sequential emergence and subsequent degeneration of clusters of cells. These observations suggest that the primary targets of interleukin 3 and granulocyte/macrophage-colony-stimulating factor are multipotent progenitors at the early stages of development rather than cells in the terminal process of maturation.
我们使用了一种用于富集人类造血祖细胞的无血清培养系统，以分析人类造血集落刺激因子的发育阶段和谱系特异性。单独的任何一种因子都不能有效地支持造血集落形成。白细胞介素3以及粒细胞/巨噬细胞集落刺激因子单独或联合使用时，均不能有效地支持祖细胞的增殖。然而，当与粒细胞集落刺激因子或促红细胞生成素联合使用时，这些因子分别产生了中性粒细胞集落或红系爆式集落。对白细胞介素3支持的培养物进行连续观察，发现细胞簇依次出现并随后退化。这些观察结果表明，白细胞介素3和粒细胞/巨噬细胞集落刺激因子的主要靶标是发育早期的多能祖细胞，而不是成熟终末阶段的细胞。
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