State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Plant J. 2020 Nov;104(4):1105-1116. doi: 10.1111/tpj.14984. Epub 2020 Sep 28.
Root nutation indicates the behavior that roots grow in a waving and skewing way due to unequal growth rates on different sides. Although a few developmental and environmental factors have been reported, genetic pathways mediating this process are obscure. We report here that the Arabidopsis CrRLK1L family member FERONIA (FER) is critical for root nutation. Functional loss of FER resulted in enhanced root waviness on tilted plates or roots forming anti-clockwise coils on horizontal plates. Suppressing polar auxin transport, either by pharmacological treatment or by introducing mutations at PIN-FORMED2 (PIN2) or AUXIN RESISTANT1 (AUX1), suppressed the asymmetric root growth (ARG) in fer-4, a null mutant of FER, indicating that FER suppression of ARG depends on polar auxin transport. We further showed by pharmacological treatments that dynamic microtubule organization and Ca signaling are both critical for FER-mediated ARG. Results presented here demonstrate a key role of FER in mediating root nutating growth, through PIN2- and AUX1-mediated auxin transport, through dynamic microtubule organization, and through Ca signaling.
根扭转是指由于不同侧面生长速度不均，根呈波浪状和扭曲状生长的行为。尽管已经报道了一些发育和环境因素，但介导这一过程的遗传途径尚不清楚。我们在这里报告称，拟南芥 CrRLK1L 家族成员 FERONIA（FER）对于根扭转至关重要。FER 的功能丧失导致在倾斜平板上根的波纹增强，或者在水平平板上根形成逆时针螺旋。通过药理学处理或在 PIN-FORMED2（PIN2）或 AUXIN RESISTANT1（AUX1）处引入突变来抑制极性生长素运输，均抑制了 fer-4（FER 的 null 突变体）中不对称根生长（ARG），表明 FER 对 ARG 的抑制依赖于极性生长素运输。我们还通过药理学处理进一步表明，动态微管组织和 Ca 信号对于 FER 介导的 ARG 都是关键的。这里呈现的结果表明，FER 通过 PIN2 和 AUX1 介导的生长素运输、通过动态微管组织和通过 Ca 信号转导，在介导根扭转生长中起关键作用。
