School of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
College of Geo-Exploration Science and Technology, Jilin University, Changchun, 130026, China.
Anal Sci. 2021 Jul 10;37(7):941-947. doi: 10.2116/analsci.20P081. Epub 2020 Sep 4.
Recently, nanozymes have become a topic of particular interest due to their high activity level, stability and biocompatibility. In this study, a visual, sensitive and selective point-of-care immunosensor was established to test the pathogen Escherichia coli O157:H7 (E. coli O157:H7). Hemin and magainin I (MI) hybrid nanocomposites (Hemin@MI) with peroxidase-mimicking activities were synthesized via a "one-pot" method, involving the simple mixing of an antimicrobial peptide (MI) against E. coli O157:H7 and hemin in a copper sulfate sodium phosphate saline buffer. Hemin@MI nanocomposites integrating target recognition and signal amplification were developed as signal probes for the point-of-care colorimetric detection of pathogenic E. coli O157:H7. Hemin@MI nanocomposites exhibit excellent peroxidase activity for the chromogenic reaction of ABTS, which allows for the visual point-of-care testing of E. coli O157:H7 in the range of 10 to 10 CFU/mL, with a limit of detection of 85 CFU/mL. These data suggest this immunosensor provides accessible and portable assessments of pathogenic E. coli O157:H7 in real samples.
最近，由于纳米酶具有高活性、稳定性和生物相容性，因此成为一个特别引人关注的话题。本研究建立了一种可视化、敏感和选择性的即时检测免疫传感器，用于检测病原体大肠杆菌 O157:H7（E. coli O157:H7）。通过“一锅法”合成了具有过氧化物酶模拟活性的血红素和抗菌肽（MI）杂化纳米复合材料（Hemin@MI），该方法涉及在硫酸铜-磷酸氢二钠盐缓冲液中简单混合针对大肠杆菌 O157:H7 的抗菌肽（MI）和血红素。血红素@MI 纳米复合材料作为信号探针，用于即时比色检测致病性大肠杆菌 O157:H7，将目标识别和信号放大结合在一起。血红素@MI 纳米复合材料对 ABTS 的显色反应具有优异的过氧化物酶活性，允许在 10 到 10 CFU/mL 的范围内进行可视化即时检测，检测限为 85 CFU/mL。这些数据表明，该免疫传感器可用于实际样本中对致病性大肠杆菌 O157:H7 进行便捷和便携的评估。
