Duan M, Han Z H, Huang T, Yang Y, Huang B
Department of Neonate, The Third Affiliated Hospital of Zunyi Medical University/The First People's Hospital of Zunyi, Zunyi, China.
Department of Emergency, The Third Affiliated Hospital of Zunyi Medical University/The First People's Hospital of Zunyi, Zunyi, China.
Lett Appl Microbiol. 2021 Jan;72(1):60-67. doi: 10.1111/lam.13382. Epub 2020 Oct 7.
This study aims to investigate the gut microbiota and metabolites in breastfed infants with breast milk jaundice (BMJ) using gut microbiome-metabolomics. Breastfed newborns diagnosed with BMJ and those without BMJ (control group) were enrolled. Faecal samples were collected from the participants and subjected to high-throughput sequencing of the 16s rDNA V3 and V4 regions of the gut flora and metabolomics of short-chain fatty acids (SCFAs). Proteobacteria, Fimicutes and Actinobacteria were the main bacteria at the phylum level. Eshcerichia-Shigella and Enterobacteriacea were the main bacteria at the genus level. The difference between the two groups was compared. Compared to the control group, the amount of Streptococcus was significantly increased while the amount of Enterococcus was significantly decreased in the faeces from infants with BMJ. Functional prediction analysis of 16S found that biosynthesis of penicillin and cephalosporin significantly increased in the BMJ group. Gas chromatography-mass spectrometry detection of SCFAs revealed that levels of acetic acid and propionic acid were significantly lower in the BMJ group than in the control group. The reduced levels of acetic acid and propionic acid may be related to the increase in Streptococcus and decrease in Enterococcus, both of which may contribute to BMJ.
本研究旨在利用肠道微生物组代谢组学研究母乳性黄疸（BMJ）母乳喂养婴儿的肠道微生物群和代谢产物。纳入诊断为BMJ的母乳喂养新生儿和未患BMJ的新生儿（对照组）。从参与者中收集粪便样本，并对肠道菌群的16s rDNA V3和V4区域进行高通量测序以及对短链脂肪酸（SCFA）进行代谢组学分析。变形菌门、厚壁菌门和放线菌门是门水平上的主要细菌。埃希氏菌-志贺氏菌属和肠杆菌科是属水平上的主要细菌。比较两组之间的差异。与对照组相比，BMJ婴儿粪便中链球菌数量显著增加，而肠球菌数量显著减少。16S功能预测分析发现，BMJ组中青霉素和头孢菌素的生物合成显著增加。气相色谱-质谱法检测SCFA显示，BMJ组中乙酸和丙酸水平显著低于对照组。乙酸和丙酸水平降低可能与链球菌增加和肠球菌减少有关，二者都可能导致BMJ。
