Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Scientific Research Center Bioclinicum, Moscow, Russia.
Dokl Biochem Biophys. 2020 Jul;493(1):198-200. doi: 10.1134/S1607672920040080. Epub 2020 Sep 7.
The role of proteasome proteins and proteins of the ERAD system in the cytotoxicity of type II ribosome-inactivating proteins ricin and viscumin was investigated. For this, the cell line of colorectal adenocarcinoma HT29, as well as the HT29-sh002 line obtained on its basis, were used. On the basis on the proteome analysis of these lines and the estimation of the proportion of inactivated ribosomes, it was shown that the contribution of the proteasome to the degradation of the catalytic subunits of toxins is different. The role of the Cdc37 co-chaperone in maintaining the stability of A subunit of viscumin in the cytoplasm is shown.
研究了蛋白酶体蛋白和 ERAD 系统蛋白在 II 型核糖体失活蛋白蓖麻毒素和相思豆毒素细胞毒性中的作用。为此，使用了结直肠腺癌 HT29 细胞系以及在此基础上获得的 HT29-sh002 系。基于这些系的蛋白质组分析和失活核糖体比例的估计，表明蛋白酶体对毒素催化亚基降解的贡献不同。还表明 Cdc37 共伴侣在保持相思豆毒素 A 亚基在细胞质中稳定性方面的作用。
