Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Scientific Research Center Bioclinicum, Moscow, Russia.
Dokl Biochem Biophys. 2020 Sep;494(1):219-221. doi: 10.1134/S1607672920040092. Epub 2020 Oct 20.
In the present study, we assessed the role of annexin 13 membrane-binding protein (ANXA13) in the intracellular transport of vesicles containing type II ribosome-inactivating proteins (RIP-IIs). A modified human intestinal epithelial cell line HT29 was used, in which the expression of ANXA13 was significantly reduced. The cytotoxic effect of ricin and viscumin was evaluated by modification of 28S ribosome RNA. The observed differences in the activity of toxins on the parental and modified HT29 lines indicate that ANXA13 plays a different role in the intracellular transport of vesicles containing the RIP-IIs.
在本研究中，我们评估了膜结合蛋白 annexin 13（ANXA13）在含有 II 型核糖体失活蛋白（RIP-II）的囊泡细胞内运输中的作用。我们使用了一种经过修饰的人肠上皮细胞系 HT29，其中 ANXA13 的表达显著降低。通过对 28S 核糖体 RNA 的修饰来评估蓖麻毒素和相思豆毒素的细胞毒性作用。在亲本和修饰的 HT29 系中观察到毒素活性的差异表明，ANXA13 在含有 RIP-II 的囊泡的细胞内运输中发挥不同的作用。
