从不同来源分离的沙门氏菌的 CRISPR 分型。
CRISPR Typing of Salmonella Isolates from Different Resources.
机构信息
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.
出版信息
Methods Mol Biol. 2021;2182:45-50. doi: 10.1007/978-1-0716-0791-6_6.
CRISPR typing is a newly developed method used to reveal the genetic relationship of bacterial isolates from different resources. For Salmonella, CRISPR typing can not only reveal the phylogenic difference among isolates belonging to the identical serotype, but also show good correspondence with Salmonella serotypes. Here we describe the protocol of CRISPR typing method used in Salmonella, and the approaches to analyze the genetic relationship among different strains.
CRISPR 分型是一种新开发的方法，用于揭示来自不同资源的细菌分离株之间的遗传关系。对于沙门氏菌，CRISPR 分型不仅可以揭示同一血清型的分离株之间的系统发育差异，而且与沙门氏菌血清型也有很好的对应关系。本文介绍了沙门氏菌 CRISPR 分型方法的实验步骤，以及分析不同菌株间遗传关系的方法。
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