Grup de Sensors i Biosensors, Departament de Química, Universitat Autònoma de Barcelona, Barcelona, Spain.
Methods Mol Biol. 2021;2182:51-65. doi: 10.1007/978-1-0716-0791-6_7.
One of the main drawbacks in current methods for bacterium detection is their quantification at very low concentration level in complex specimens. Novel developments that are needed involve solid-phase preconcentration procedures which can be easily integrated with emerging technologies. Here, we describe the immunomagnetic separation (IMS) of Salmonella using magnetic carriers. Nano (300 nm) and micro (2.8 μm) sized magnetic particles are modified with anti-Salmonella antibody to preconcentrate the bacteria from the samples throughout an immunological reaction. The immunomagnetic separation can be easily coupled with downstream characterization and quantification methods, including classical culturing, molecular biology techniques such as PCR, immunoassays, confocal and scanning electron microscopy, and emerging technologies and rapid detection methods including biosensors, lateral flow, and microfluidic devices.
目前用于细菌检测的方法主要存在一个缺陷，即它们无法在复杂样本中对非常低浓度的细菌进行定量。需要新的发展，涉及固相预浓缩程序，该程序可以与新兴技术轻松集成。在这里，我们描述了使用磁性载体对沙门氏菌进行免疫磁分离 (IMS)。纳米（300nm）和微（2.8μm）尺寸的磁性颗粒用抗沙门氏菌抗体修饰，以通过免疫反应从样品中预浓缩细菌。免疫磁分离可以很容易地与下游的表征和定量方法结合使用，包括经典培养、聚合酶链反应等分子生物学技术、免疫测定、共聚焦和扫描电子显微镜，以及新兴技术和快速检测方法，包括生物传感器、侧向流动和微流控装置。
