Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming, China.
J Clin Lab Anal. 2020 Sep;34(9):e23417. doi: 10.1002/jcla.23417. Epub 2020 Sep 8.
In clinical practice, the cases with bacterial infection caused by titanium implants and bacterial biofilm formation on the surface of titanium materials implanted into human body can often be observed. Thus, this study aimed to demonstrate whether the mixed biofilm of Staphylococcus aureus/Escherichia coli can be formed on the surface of titanium material through in vitro experiments and its formation rules.
The titanium plates were put into the well containing S aureus or/and E coli. Bacterial adhesion and biofilm formation were analyzed by crystal violet, XTT method, confocal laser scanning microscopy, and scanning electron microscopy.
The results of bacterial adhesion in each group at 6-72 hours showed that the number of bacterial adhesion in each group was increased with the extension of time and reached to the highest level at 72 hours. Moreover, the biofilm structure in the S aureus-E coli group was significantly more complex than that of the simple S aureus group or E coli group, and the number of bacteria was also significantly increased in the S aureus-E coli group.
Those data provide a laboratory basis for the prevention and treatment of mixed infection of subsequent biological materials.
在临床实践中，经常可以观察到钛植入物引起的细菌感染病例和钛材料表面细菌生物膜的形成。因此，本研究旨在通过体外实验证明金黄色葡萄球菌/大肠杆菌混合生物膜是否可以在钛材料表面形成及其形成规律。
将钛板放入含有金黄色葡萄球菌或/和大肠杆菌的孔中。通过结晶紫、XTT 法、共聚焦激光扫描显微镜和扫描电子显微镜分析细菌黏附与生物膜形成情况。
各实验组在 6-72 小时的细菌黏附结果显示，各实验组的细菌黏附数量随时间的延长而增加，在 72 小时达到最高水平。此外，金黄色葡萄球菌-大肠杆菌组的生物膜结构明显比单纯金黄色葡萄球菌组或大肠杆菌组复杂，且金黄色葡萄球菌-大肠杆菌组的细菌数量也明显增加。
这些数据为后续生物材料混合感染的预防和治疗提供了实验室依据。
