Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, U.S.A.
Howard Hughes Medical Institute, Basic Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, U.S.A.
Biochem Soc Trans. 2020 Oct 30;48(5):1967-1978. doi: 10.1042/BST20191226.
RNA plays a well-established architectural role in the formation of membraneless interchromatin nuclear bodies. However, a less well-known role of RNA is in organizing chromatin, whereby specific RNAs have been found to recruit chromatin modifier proteins. Whether or not RNA can act as an architectural molecule for chromatin remains unclear, partly because dissecting the architectural role of RNA from its regulatory role remains challenging. Studies that have addressed RNA's architectural role in chromatin organization rely on in situ RNA depletion using Ribonuclease A (RNase A) and suggest that RNA plays a major direct architectural role in chromatin organization. In this review, we will discuss these findings, candidate chromatin architectural long non-coding RNAs and possible mechanisms by which RNA, along with RNA binding proteins might be mediating chromatin organization.
RNA 在形成无膜核间核体中起着成熟的结构作用。然而，RNA 的一个不太为人知的作用是组织染色质，特定的 RNA 已被发现招募染色质修饰蛋白。RNA 是否可以作为染色质的结构分子尚不清楚，部分原因是从调节作用中分离 RNA 的结构作用仍然具有挑战性。已经解决了 RNA 在染色质组织中的结构作用的研究依赖于使用核糖核酸酶 A (RNase A) 进行原位 RNA 耗竭，并表明 RNA 在染色质组织中起着主要的直接结构作用。在这篇综述中，我们将讨论这些发现、候选染色质结构长非编码 RNA 以及 RNA 与 RNA 结合蛋白可能介导染色质组织的可能机制。
