Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to Shandong University , Jinan, Shandong, China.
Department of Cardiac Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University , Jinan, Shandong, China.
Cell Cycle. 2020 Oct;19(20):2622-2630. doi: 10.1080/15384101.2020.1814025. Epub 2020 Sep 8.
Myocardial infarction (MI) is the main cause of morbidity and mortality. Reperfusion ways can cause damage to cardiomyocytes. CircMAT2B, a novel circRNA, takes positive roles in regulating glucose metabolism under hypoxia. Therefore, we aimed to explore the effects of circMAT2B on MI. Oxygen-glucose deprivation (OGD)-induced H9c2 cell model was employed to stimulate MI. Ex-circMAT2B, si-circMAT2B, miR-133 inhibitor and relative control were transfected into H9c2 cells. qRT-PCR was employed to examine levels of circMAT2B and miR-133. Cell activity, apoptosis, ROS generation and release of inflammatory factors were assessed by CCK-8, flow cytometry, ROS species assay kit and ELISA, respectively. Moreover, the expression of apoptosis-related and pathway-related factors was detected through western blot analysis. The results showed that circMAT2B expression was notably up-regulated by OGD treatment. Moreover, circMAT2B knockdown could effectively decrease OGD-induced the increasing of apoptosis, ROS generation and the expression of IL-1β, IL-6 and TNF-α. Besides, miR-133 was positively regulated by si-circMAT2B. CircMAT2B knockdown attenuated OGD-induced H9c2 cell damage and alleviated OGD-induced the inhibition of PI3K/AKT and Raf/MEK/ERK pathways through up-regulating miR-133. In brief, circMAT2B knockdown works as an inflammatory inhibitor in OGD-induced H9c2 cells inflammatory injury through up-regulating miR-133.
心肌梗死（MI）是发病率和死亡率的主要原因。再灌注方式可导致心肌细胞损伤。CircMAT2B 是一种新型的 circRNA，在缺氧下对调节葡萄糖代谢具有积极作用。因此，我们旨在探讨 circMAT2B 对 MI 的影响。采用氧葡萄糖剥夺（OGD）诱导的 H9c2 细胞模型刺激 MI。将 Ex-circMAT2B、si-circMAT2B、miR-133 抑制剂和相对对照转染到 H9c2 细胞中。qRT-PCR 用于检测 circMAT2B 和 miR-133 的水平。通过 CCK-8、流式细胞术、ROS 物种测定试剂盒和 ELISA 分别评估细胞活性、细胞凋亡、ROS 生成和炎症因子的释放。此外，通过 Western blot 分析检测凋亡相关和通路相关因子的表达。结果表明，OGD 处理显著上调了 circMAT2B 的表达。此外，circMAT2B 敲低可有效降低 OGD 诱导的细胞凋亡增加、ROS 生成增加以及 IL-1β、IL-6 和 TNF-α 的表达增加。此外，miR-133 被 si-circMAT2B 正向调节。circMAT2B 敲低通过上调 miR-133 减轻 OGD 诱导的 H9c2 细胞损伤，并缓解 OGD 诱导的 PI3K/AKT 和 Raf/MEK/ERK 通路抑制。总之，circMAT2B 敲低通过上调 miR-133 在 OGD 诱导的 H9c2 细胞炎症损伤中发挥炎症抑制剂的作用。
