College of Life Science, Henan Normal University, Xinxiang, 453007, China; State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, 453007, China.
College of Life Science, Henan Normal University, Xinxiang, 453007, China; State Key Laboratory Cultivation Base for Cell Differentiation Regulation, Henan Normal University, Xinxiang, 453007, China.
Arch Biochem Biophys. 2020 Oct 30;693:108567. doi: 10.1016/j.abb.2020.108567. Epub 2020 Sep 6.
By comparing differentially abundant proteins and metabolites, the protein expression, metabolic changes and metabolic regulation mechanisms during the priming phase of liver regeneration (LR) were investigated. We combined proteomic analysis via isobaric tags for relative and absolute quantification (iTRAQ) with metabolomic analysis via nontargeted liquid chromatography-mass spectrometry (LC-MS). LC-MS was used to examine 29 energy metabolites expression alterations in targeted metabolomics. A total number of 441 differentially expressed proteins and 65 metabolites were identified. PSMB10, PSMB5, RCG_63409, PSME4 and PSMB7 were key node proteins, these proteins are involved in the proteasome pathway. The most strongly enriched transcription factor motif was TP63. These results point out a critical role of the proteasome pathway (defense mechanisms) and of TP63 (metabolic regulator) as the key transcription factor during the priming phase of LR. Metabolomic and metabolite analysis showed that profiling indicates upregulation of arginine biosynthesis and glycolysis as the main ATP-delivering pathway. Integrative proteomic and metabolomic analysis showed that biomolecular changes were primarily related to the neurological disease, cell death and survival and cell morphology. What's more, neurotransmitters may play an important role in the regulation of LR.
通过比较差异丰度的蛋白质和代谢物，研究了肝再生（LR）启动阶段的蛋白质表达、代谢变化和代谢调控机制。我们将相对和绝对定量标记（iTRAQ）的蛋白质组学分析与非靶向液相色谱-质谱（LC-MS）的代谢组学分析相结合。LC-MS 用于检测靶向代谢组学中 29 种能量代谢物表达的改变。共鉴定到 441 种差异表达蛋白和 65 种代谢物。PSMB10、PSMB5、RCG_63409、PSME4 和 PSMB7 是关键节点蛋白，这些蛋白参与蛋白酶体途径。最富集的转录因子基序是 TP63。这些结果表明，在 LR 的启动阶段，蛋白酶体途径（防御机制）和 TP63（代谢调节剂）作为关键转录因子起着关键作用。代谢组学和代谢物分析表明，谱分析表明精氨酸生物合成和糖酵解的上调是主要的 ATP 供应途径。综合蛋白质组学和代谢组学分析表明，生物分子变化主要与神经疾病、细胞死亡和存活以及细胞形态有关。此外，神经递质可能在 LR 的调节中发挥重要作用。
