Higgins Paul G, Hagen Ralf Matthias, Podbielski Andreas, Frickmann Hagen, Warnke Philipp
Institute for Medical Microbiology, Immunology, and Hygiene, University of Cologne, 50935 Cologne, Germany.
German Centre for Infection Research (DZIF), partner site Bonn-Cologne, 50935 Cologne, Germany.
Antibiotics (Basel). 2020 Sep 5;9(9):579. doi: 10.3390/antibiotics9090579.
Recently, a total of 32 carbapenem- and fluoroquinolone-resistant (Ab) isolates was isolated from war-injured patients who were treated at German Bundeswehr Hospitals, and preliminarily typed by "DiversiLab" repetitive elements sequence-based (rep-) PCR. Core genome-based sequence typing was also used to provide more detailed epidemiological information. From the clusters observed by rep-PCR, selected Ab strains were subjected to Next Generation Sequencing (NGS) in order to compare them with international outbreak-associated Ab strains and to identify MLST (multi-locus sequence type) lineages, as well as to identify known resistance genes. Accordingly, NGS indicated higher diversity than rep-PCR, but also confirmed likely transmission events. The identified acquired carbapenem-resistant genes comprised , and , as well as various other intrinsic and acquired resistance-associated genetic elements. All isolates clustered with the previously identified international clonal lineages IC1, IC2, IC6 and IC7, with corresponding Pasteur sequence types ST1, ST2, ST78 and ST25, respectively. In conclusion, the assessment confirmed a broad spectrum of resistance-associated genes in Ab isolated from war-injured patients from the Eastern Ukraine, and provided the first insights into locally abundant clonal lineages.
最近，从在德国联邦国防军医院接受治疗的战争伤员中总共分离出32株对碳青霉烯类和氟喹诺酮耐药的（Ab）菌株，并通过基于“DiversiLab”重复元件序列（rep-）PCR进行初步分型。基于核心基因组的序列分型也被用于提供更详细的流行病学信息。从rep-PCR观察到的聚类中，选择的Ab菌株进行了下一代测序（NGS），以便与国际暴发相关的Ab菌株进行比较，并确定多位点序列分型（MLST）谱系，以及识别已知的耐药基因。因此，NGS显示出比rep-PCR更高的多样性，但也证实了可能的传播事件。鉴定出的获得性碳青霉烯耐药基因包括、和，以及各种其他固有和获得性耐药相关的遗传元件。所有分离株分别与先前鉴定的国际克隆谱系IC1、IC2、IC6和IC7聚类，对应的巴斯德序列类型分别为ST1、ST2、ST78和ST25。总之，评估证实了从乌克兰东部战争伤员中分离出的Ab中存在广泛的耐药相关基因，并首次深入了解了当地丰富的克隆谱系。
