López-Hernández Inmaculada, García Barrionuevo Aurora, Díaz de Alba Paula, Clavijo Encarnación, Pascual Alvaro
Laboratorio PIRASOA, Unidad Clínica de Enfermedades Infecciosas, Microbiología y Medicina Preventiva, Hospital Universitario Virgen Macarena, Seville, Spain; Instituto de Biomedicina de Sevilla (IBiS), Seville, Spain.
Servicio de Microbiología, Hospital Virgen de la Victoria, Málaga, Spain.
Enferm Infecc Microbiol Clin (Engl Ed). 2020 Aug-Sep;38(7):327-330. doi: 10.1016/j.eimc.2019.10.007. Epub 2019 Dec 6.
To characterize a carbapenem-resistant Enterobacter cloacae complex isolate recovered from a patient from Ukraine.
The isolate was sent to a regional reference laboratory for molecular characterization by whole genome sequencing. Susceptibility assays, carbapenemase identification, imipenem hydrolysis and clonality were performed.
The isolate showed resistance or reduced susceptibility to all β-lactam agents tested. Genome analysis led to the identification of an NDM-1-producing E. cloacae complex strain that was assigned to a new multilocus sequence type, ST932. The bla enzyme was located in a conjugative IncX3 plasmid of ca. 50kb. In addition, bla, a recently described AmpC β-lactamase sequence, which has not previously been reported in Europe, was also detected and its genetic environment was studied.
To our knowledge, this is the first reported case in Europe of an E. cloacae complex strain that produces both bla and bla.
对从一名乌克兰患者身上分离出的耐碳青霉烯类阴沟肠杆菌复合菌进行特征描述。
将该分离株送至区域参考实验室，通过全基因组测序进行分子特征分析。进行了药敏试验、碳青霉烯酶鉴定、亚胺培南水解试验和克隆性分析。
该分离株对所有测试的β-内酰胺类药物均表现出耐药性或敏感性降低。基因组分析鉴定出一株产NDM-1的阴沟肠杆菌复合菌菌株，该菌株被归类为一种新的多位点序列类型，ST932。bla酶位于一个约50kb的接合型IncX3质粒上。此外，还检测到一种最近描述的AmpCβ-内酰胺酶序列bla，此前在欧洲尚未有报道，并对其遗传环境进行了研究。
据我们所知，这是欧洲首次报道的一株同时产生bla和bla的阴沟肠杆菌复合菌菌株病例。
