Magaña-Dueñas Viridiana, Stchigel Alberto M, Cano-Lira José F
Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili, Sant Llorenç 21, 43201 Reus, Tarragona, Spain.
Microorganisms. 2020 Sep 4;8(9):1355. doi: 10.3390/microorganisms8091355.
With the exception of the so-called Ingoldian fungi, the diversity and distribution of the freshwater aero-aquatic or facultative fungi are not well known in Spain. In view of that, we collected and placed into wet chambers 105 samples of submerged and decomposing plant debris from various places in Spain, looking for individuals belonging to these latter two morpho-ecological groups of fungi. As a result, we found and isolated in pure culture several fungi, the morphology of some of them belonging to the family (order , class ). After a careful phenotypic characterization and a phylogenetic tree reconstruction using a concatenated sequence dataset of D1-D2 domains of the 28S nrRNA gene (), the internal transcribed spacer region (ITS) of the nrDNA, and a fragment of the translation elongation factor 1-alpha () gene, we report the finding of three new species of the genus : , which produces cinnamon-colored, broadly fusiform to navicular ascospores; , which has as a remarkable characteristic the production of both sexual and asexual morphs in vitro; and , the unique species of the genus that lacks a sexual morph. As a consequence of the phylogenetic study, we introduce the new aero-aquatic genus , with a new combination (, formerly as the type species of the genus) and a new species, ; we propose the new combinations (formerly ) and (formerly ); and we resurrect , which is transferred to the family Lopiostomataceae.
除了所谓的英戈尔迪真菌外，西班牙对淡水气生-水生或兼性真菌的多样性和分布了解甚少。鉴于此，我们收集了来自西班牙各地的105份淹没且正在分解的植物残体样本，并将其置于湿室中，寻找属于后两种形态-生态真菌类群的个体。结果，我们发现并在纯培养中分离出了几种真菌，其中一些真菌的形态属于（目，纲）科。在对28S nrRNA基因的D1-D2结构域（）、nrDNA的内部转录间隔区（ITS）以及翻译延伸因子1-α（）基因的片段进行串联序列数据集的仔细表型特征分析和系统发育树重建后，我们报告了属的三个新物种的发现：，其产生肉桂色、宽梭形至舟形的子囊孢子；，其显著特征是在体外产生有性和无性形态；以及，该属中唯一缺乏有性形态的物种。作为系统发育研究的结果，我们引入了新的气生-水生属，以及一个新组合（，以前作为该属的模式种）和一个新物种，；我们提出了新组合（以前为）和（以前为）；并且我们复活了，它被转移到洛皮奥斯托马科。
