Magaña-Dueñas Viridiana, Cano-Lira José Francisco, Stchigel Alberto Miguel
Mycology Unit, Medical School, Universitat Rovira i Virgili, C/Sant Llorenç 21, 43201 Reus, Spain.
J Fungi (Basel). 2022 Aug 14;8(8):849. doi: 10.3390/jof8080849.
Freshwater ascomycetes are a group of fungi of great ecological importance because they are involved in decomposition processes and the recycling of organic matter in aquatic ecosystems. The taxonomy of these fungi is complex, with representatives in several orders of the phylum Ascomycota. In the present study, we collected ninety-two samples of plant debris submerged in freshwater in different locations in Spain. The plant specimens were placed in wet chambers and developed several fungi that were later isolated in pure culture. A main phylogenetic tree using the nucleotide sequences of D1-D2 domains of the 28S nrRNA gene (LSU) was built to show the taxonomic placement of all our fungal strains, and, later, individual phylogenies for the different families were built using single or concatenated nucleotide sequences of the most suitable molecular markers. As a result, we found a new species of that produces a coelomycetous asexual state, a new species of that produces an asexual state, a new species of that forms both sexual and asexual states in vitro, and the sexual states of two species of none of which have been reported before for these genera. In addition, we describe a new species of characterized by the production of copper-colored globose conidiomata, and of , which produces well-developed conidiophores.
淡水子囊菌是一类具有重要生态意义的真菌，因为它们参与水生生态系统中的分解过程和有机物质的循环。这些真菌的分类很复杂，在子囊菌门的几个目中都有代表。在本研究中，我们在西班牙不同地点收集了92份淹没在淡水中的植物残体样本。将植物标本置于湿室中，培养出了几种真菌，随后将其分离为纯培养物。构建了一个使用28S nrRNA基因（LSU）D1-D2结构域核苷酸序列的主要系统发育树，以显示我们所有真菌菌株的分类位置，随后，使用最合适分子标记的单个或串联核苷酸序列构建了不同科的个体系统发育树。结果，我们发现了一种产生腔孢型无性态的新物种，一种产生无性态的新物种，一种在体外形成有性和无性态的新物种，以及两个物种的有性态，这些属之前均未报道过这些情况。此外，我们描述了一种以产生铜色球形分生孢子器为特征的新物种，以及一种产生发育良好分生孢子梗的新物种。
