University of Lyon, UJM-Saint-Etienne, CNRS, EVS-ISTHME UMR 5600, F-42023, Saint-Etienne, France.
Laboratoire Chrono-Environnement - UMR 6249, Université de Bourgogne Franche-Comté, 16, route de Gray, 25 000, Besançon, France.
Environ Sci Pollut Res Int. 2021 Jan;28(3):2987-2994. doi: 10.1007/s11356-020-10681-8. Epub 2020 Sep 8.
In La Glacière cave (France), the touristic activity has been conducted to an environmental parameter change that has led to photosynthetic organism proliferation (microalgae, diatoms, cyanobacteria, bryophytes). The present study is focused on bryophyte development occurring in the show cave that was responsible of limestone biodeterioration. In order to understand the colonization process of limestone, we have maintained limestone blocks under optimal Lampenflora growth conditions. Moreover, some limestone blocks were painted with several pigments that were used in the prehistory (e.g., red ocher, bone char). Microorganisms and bryophyte growth were monitored during 1 year, and then, the block samples were treated using UV-C light (254 nm). Thus, obtained results were compared with in situ treatment in La Glacière cave. Results have showed dense bryophyte propagation on the several blocks. However, the growth rate was correlated with the chemical composition of the pigment. In fact, the presence of some chemical elements such as As, Cr, Ti, and Co contributed to reduce bryophyte growth. Finally, moss treatment using UV-C light has demonstrated high efficiency under in situ condition, while a fast recolonization has been observed for samples maintained in laboratory. This difference was explained by the high bryophyte density under laboratory conditions that make UV-C light penetration difficult.
在法国的拉冰川洞穴（La Glacière cave）中，旅游活动导致了环境参数的变化，从而导致了光合生物的增殖（微藻、硅藻、蓝藻、苔藓植物）。本研究集中于洞穴中苔藓植物的发育，这是导致石灰岩生物降解的原因。为了了解石灰岩的定殖过程，我们将石灰岩块保存在最佳的 Lampenflora 生长条件下。此外，一些石灰岩块被涂上了几种史前使用的颜料（如红赭石、骨炭）。在一年的时间里，监测了微生物和苔藓植物的生长情况，然后用 254nm 的 UV-C 光对块状物进行了处理。因此，将获得的结果与拉冰川洞穴中的原位处理进行了比较。结果表明，几块石头上苔藓植物密集繁殖。然而，生长速度与颜料的化学成分有关。事实上，一些化学元素的存在，如砷、铬、钛和钴，有助于减少苔藓植物的生长。最后，用 UV-C 光对苔藓进行处理，在原位条件下显示出了很高的效率，而在实验室中保存的样本则观察到了快速的再定殖。这种差异是由实验室条件下苔藓植物密度高造成的，这使得 UV-C 光难以穿透。
