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构建用于二氢叶酸还原酶诱变实验的大肠杆菌叶酸突变株。

Construction of a fol mutant strain of Escherichia coli for use in dihydrofolate reductase mutagenesis experiments.

作者信息

Ahrweiler P M, Frieden C

机构信息

Department of Biochemistry, Washington University Medical School, St. Louis, Missouri 63110.

出版信息

J Bacteriol. 1988 Jul;170(7):3301-4. doi: 10.1128/jb.170.7.3301-3304.1988.

DOI:10.1128/jb.170.7.3301-3304.1988
PMID:3290203
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211288/
Abstract

A strain of Escherichia coli with the fol gene deleted and a kan gene inserted in its place was created for use in cloning and isolation of mutant dihydrofolate reductase. Southern blot analysis and dihydrofolate reductase enzyme assays confirmed the delta fol::kan genotype. A thyA mutation accompanied the fol deletion and is required for survival of a dihydrofolate reductase-deficient strain.

摘要

构建了一株缺失fol基因并用kan基因取代的大肠杆菌菌株，用于克隆和分离突变型二氢叶酸还原酶。Southern印迹分析和二氢叶酸还原酶酶活性测定证实了delta fol::kan基因型。thyA突变伴随fol缺失，是二氢叶酸还原酶缺陷型菌株存活所必需的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a1/211288/144a6a08b6d6/jbacter00185-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a1/211288/144a6a08b6d6/jbacter00185-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0a1/211288/144a6a08b6d6/jbacter00185-0423-a.jpg

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Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
Microbiol Rev. 1983 Jun;47(2):180-230. doi: 10.1128/mr.47.2.180-230.1983.
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Characterization of the Escherichia coli thyA gene and its amplified thymidylate synthetase product.大肠杆菌thyA基因及其扩增的胸苷酸合成酶产物的特性分析。
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Genomic sequencing.基因组测序
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