School of Chemistry and Molecular Engineering, East China University of Science and Technology , Shanghai, China.
School of Life Sciences, Shanghai University , Shanghai, China.
Plant Signal Behav. 2020 Dec 1;15(12):1816322. doi: 10.1080/15592324.2020.1816322. Epub 2020 Sep 9.
Heat stress is a major growth-limiting factor for most crops over the world. Chitin elicitor receptor kinase 1 () is a chitin/chitooligosaccharides receptor, and () plays a crucial role in plant resistance to heat stress. In the present study, a chitooligosaccharides-induced fusion gene was designed and synthesized, in which the extracellular domain and transmembrane domain of gene is connected with the response region of gene. We successfully constructed the fusion gene by Overlap PCR and introduced it into by infection. Genetically modified (GM) plants had a greater germination rate and germination index, as well as a shorter mean germination time, indicating that they had a better thermotolerance compared with the wild-type (WT) lines under heat stress. Moreover, the GM lines showed a lower level of hydrogen peroxide (HO) and relative electrolyte leakage (REL), suggesting that they were in better state compared with the WT plants when exposed to high temperature. UPLC-MS/MS was employed to assess the phytohormone level, suggesting that the GM lines acquired a better thermotolerance via jasmonic acid (JA) signaling pathways. In general, we constructed a COS-induced fusion gene to enhance the thermotolerance of during seed germination and postgermination growth.
热应激是世界上大多数作物生长的主要限制因素。几丁质激发受体激酶 1（）是一种几丁质/壳寡糖受体，在植物耐热性中发挥着关键作用。在本研究中，设计并合成了一种壳寡糖诱导的融合基因，其中基因的胞外域和跨膜域与基因的响应区域相连。我们通过重叠 PCR 成功构建了融合基因，并通过 感染将其导入 。与野生型（WT）系相比，转基因（GM）植物在热胁迫下具有更高的发芽率和发芽指数，以及更短的平均发芽时间，表明它们具有更好的耐热性。此外，GM 系表现出较低的过氧化氢（HO）和相对电解质渗漏（REL）水平，表明它们在暴露于高温时的状态比 WT 植物更好。采用 UPLC-MS/MS 评估植物激素水平，表明 GM 系通过茉莉酸（JA）信号通路获得了更好的耐热性。总的来说，我们构建了一个 COS 诱导的融合基因，以增强种子萌发和萌发后生长过程中 的耐热性。
