Kozyulina Polina Yu, Pavlova Olga A, Kantsurova Rudaya Elizaveta S, Bovin Andrey D, Shirobokova Svetlana A, Dolgikh Aleksandra V, Dymo Alina M, Dolgikh Elena A
Laboratory of Signal Regulation, All-Russia Research Institute for Agricultural Microbiology, St.-Petersburg, Russia.
Front Plant Sci. 2023 Mar 8;14:1092013. doi: 10.3389/fpls.2023.1092013. eCollection 2023.
Since chitooligosaccharides (COs) are water-soluble, biodegradable and nontoxic compounds, their application may be considered as a promising plant-protecting agent. However, the molecular and cellular modes of action of COs are not yet understood. In this study, transcriptional changes in pea roots treated with COs were investigated RNA sequencing. Pea roots treated with the deacetylated CO8-DA at low concentration (10 М) were harvested 24 h after treatment and their expression profiles were compared against medium-treated control plants. We observed 886 differentially expressed genes (fold change ≥ 1; p-value < 0.05) 24 h after treatment with CO8-DA. Gene Ontology term over-representation analysis allowed us to identify the molecular functions of the genes activated in response to CO8-DA treatment and their relation to biological processes. Our findings suggest that calcium signaling regulators and MAPK cascade play a key role in pea plant responses to treatment. Here we found two MAPKKKs, the PsMAPKKK5 and PsMAPKKK20, which might function redundantly in the CO8-DA-activated signaling pathway. In accordance with this suggestion, we showed that knockdown decreases resistance to pathogenic fungi. Therefore, analysis showed that typical regulators of intracellular signal transduction pathways involved in triggering of plant responses CERK1 receptors to chitin/COs in and rice may also be recruited in legume pea plants.
由于壳寡糖（COs）是水溶性、可生物降解且无毒的化合物，其应用可被视为一种有前景的植物保护剂。然而，壳寡糖的分子和细胞作用模式尚不清楚。在本研究中，通过RNA测序研究了用壳寡糖处理的豌豆根中的转录变化。用低浓度（10μM）的脱乙酰化壳寡糖CO8-DA处理豌豆根，在处理后24小时收获，将其表达谱与用培养基处理的对照植物进行比较。在用CO8-DA处理24小时后，我们观察到886个差异表达基因（倍数变化≥1；p值<0.05）。基因本体术语过度表达分析使我们能够确定响应CO8-DA处理而激活的基因的分子功能及其与生物学过程的关系。我们的研究结果表明，钙信号调节剂和丝裂原活化蛋白激酶（MAPK）级联在豌豆植物对处理的反应中起关键作用。在这里，我们发现了两个MAPKKK，即PsMAPKKK5和PsMAPKKK20，它们可能在CO8-DA激活的信号通路中发挥冗余作用。根据这一推测，我们表明基因敲除会降低对致病真菌的抗性。因此，分析表明，参与触发植物反应的细胞内信号转导途径的典型调节剂，如拟南芥和水稻中几丁质/壳寡糖的CERK1受体，也可能在豆科豌豆植物中被募集。
