Jia Jian, Xu Hang, Chen Changbo, Zhang Xiaoqian, Zhang Xuexue, Li Wei, Ma Jing
Department of Traditional Chinese Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, China.
Gen Physiol Biophys. 2020 Jul;39(4):331-341. doi: 10.4149/gpb_2020013.
Quantitative proteomic analysis was performed using iTRAQ to explore the potential regulation of differentially expressed proteins (DEPs) by bisphenol A (BPA) in murine testis. BPA was intraperitoneally injected into mice at a dose of 100 mg/kg body weight for 7 consecutive days. After BPA treatment, the histopathology changes of testis were examined. The circulating levels of testosterone (T) and estradiol (E2) were determined. iTRAQ was used to assess the expression levels of DEPs and to reveal potential interactions between different DEPs. Results showed that BPA caused histological damage in testicular tissues. The levels of T and E2 were affected by BPA exposure. The abundances of orosomucoid 1 (Orm1), haptoglobin (Hp), and insulin-like 3 (Insl3) were significantly lower in BPA-treated mice than those in control mice. The expression changes in the above-mentioned proteins were further validated at the protein level using Western blot analysis. We concluded that BPA affects histological morphology of testis and sex hormone productions. The regulation of key proteins (such as Orm1, Hp and Insl3) may reflect that these proteins may serve as important factors in male reproductive disorders caused by BPA, and these proteins are probably biomarkers for infertility caused by endocrine disrupting chemicals.
采用iTRAQ进行定量蛋白质组学分析，以探索双酚A（BPA）对小鼠睾丸中差异表达蛋白（DEP）的潜在调控作用。将BPA以100 mg/kg体重的剂量腹腔注射到小鼠体内，连续注射7天。BPA处理后，检查睾丸的组织病理学变化。测定睾酮（T）和雌二醇（E2）的循环水平。使用iTRAQ评估DEP的表达水平，并揭示不同DEP之间的潜在相互作用。结果表明，BPA导致睾丸组织出现组织学损伤。BPA暴露影响了T和E2的水平。在BPA处理的小鼠中，类粘蛋白1（Orm1）、触珠蛋白（Hp）和胰岛素样3（Insl3）的丰度显著低于对照小鼠。使用蛋白质印迹分析在蛋白质水平进一步验证了上述蛋白质的表达变化。我们得出结论，BPA影响睾丸的组织形态和性激素分泌。关键蛋白（如Orm1、Hp和Insl3）的调控可能反映出这些蛋白可能是BPA引起男性生殖障碍的重要因素，并且这些蛋白可能是内分泌干扰化学物质导致不育的生物标志物。
