Institute of Animal Nutrition, Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, Braunschweig, Germany.
BIOMIN Holding GmbH, BIOMIN Research Center, Tulln, Austria.
Mycotoxin Res. 2020 Nov;36(4):429-442. doi: 10.1007/s12550-020-00403-x. Epub 2020 Sep 9.
The main objective of this study was to evaluate the effects of sodium sulfite (SoS) treatment of maize and its impact on the porcine immune system in the presence of an LPS-induced systemic inflammation. Control maize (CON) and Fusarium-toxin contaminated maize (FUS) were wet-preserved (20% moisture) for 79 days with (+) or without (-) SoS and then included at 10% in a diet, resulting in four experimental groups: CON-, CON+, FUS-, and FUS+ with deoxynivalenol (DON) concentrations of 0.09, 0.05, 5.36, and 0.83 mg DON/kg feed, respectively. After 42-day feeding trial (weaned barrows, n = 20/group), ten pigs per group were challenged intraperitoneally with either 7.5 μg LPS/kg BW or placebo (0.9% NaCl), observed for 2 h, and then sacrificed. Blood, mesenteric lymph nodes, and spleen were collected for phenotyping of different T cell subsets, B cells, and monocytes. Phagocytic activity and intracellular formation of reactive oxygen species (ROS) were analyzed in both polymorphonuclear cells (PMN) and peripheral blood mononuclear cells (PBMC) using flow cytometry. Our results revealed that the impact of DON was more notable on CD3CD4CD8 T cells in lymphoid tissues rather than in blood T cells. In contrast, SoS treatment of maize altered leukocyte subpopulations in blood, e.g., reduced the percentage and fluorescence signal of CD8 T cells. Interestingly, SoS treatment reduced the amount of free radicals in basal ROS-producing PMNs only in LPS-challenged animals, suggesting a decrease in basal cellular ROS production (p = 0.022).
本研究的主要目的是评估亚硫酸钠（SoS）处理玉米及其对脂多糖（LPS）诱导的全身炎症状态下猪免疫系统的影响。对照玉米（CON）和污染有镰刀菌毒素的玉米（FUS）经湿储（20%水分）79 天，用（+）或不用（-）SoS 处理，然后以 10%的比例添加到饲料中，共得到四个实验组：CON-、CON+、FUS-和 FUS+，其脱氧雪腐镰刀菌烯醇（DON）浓度分别为 0.09、0.05、5.36 和 0.83 mg DON/kg 饲料。经过 42 天的饲养试验（断奶公猪，每组 20 头），每组 10 头猪分别经腹腔内注射 7.5 μg LPS/kg BW 或安慰剂（0.9% NaCl），观察 2 小时，然后处死。收集血液、肠系膜淋巴结和脾脏，用于不同 T 细胞亚群、B 细胞和单核细胞的表型分析。用流式细胞术分析多形核细胞（PMN）和外周血单核细胞（PBMC）中的吞噬活性和活性氧（ROS）的细胞内形成。结果表明，DON 对淋巴组织中的 CD3CD4CD8 T 细胞的影响比血液 T 细胞更为显著。相比之下，SoS 处理玉米改变了血液中的白细胞亚群，例如，减少了 CD8 T 细胞的比例和荧光信号。有趣的是，SoS 处理仅在 LPS 攻毒的动物中降低了基础 ROS 产生 PMN 中的自由基数量，表明基础细胞 ROS 产生减少（p = 0.022）。
