Yang Fengyuan, Wang Yanping, Zhao Shanshan, Wang Yuan
Henan Provincial Key Laboratory of Ion Beam Bioengineering, School of Agricultural Science, Zhengzhou University, Zhengzhou, China.
Henan Provincial Key Laboratory of Ion Beam Bioengineering, College of Physics, Zhengzhou University, Zhengzhou, China.
Front Microbiol. 2020 Aug 12;11:1989. doi: 10.3389/fmicb.2020.01989. eCollection 2020.
The objective of this study was to investigate the mechanism of () involved in improving fermentation quality of naturally ensiled alfalfa under poor conditions. High-moisture wilted alfalfa was ensiled without inoculants (CK) or with inoculation of two additives (LPI and LPII). The pH and fermentation products of silage were determined after 30 and 90 days of ensiling. Additionally, the bacterial community compositions were analyzed. The inoculants significantly promoted lactic acid accumulation, and abundance for both periods. At 90 days, silage in CK exhibited a high pH, a loss in dry matter, and a high concentration of ammoniacal nitrogen. The inoculations of significantly inhibited the growth of Clostridia, and reduced ammoniacal nitrogen concentration in silage ( < 0.05). Thus, inoculation with improved the fermentation quality of alfalfa silage and inhibited the growth of spoilage microorganisms, and further delayed spoilage of alfalfa silage under adverse ensiling conditions.
本研究的目的是探究（）在恶劣条件下改善天然青贮苜蓿发酵品质的机制。高水分萎蔫苜蓿在不添加接种剂（CK）或添加两种添加剂（LPI和LPII）的情况下进行青贮。青贮30天和90天后测定青贮饲料的pH值和发酵产物。此外，还分析了细菌群落组成。接种剂显著促进了乳酸积累以及两个时期的（）丰度。在90天时，CK组青贮饲料的pH值较高，干物质损失较大，氨态氮浓度较高。（）接种显著抑制了梭菌的生长，并降低了青贮饲料中的氨态氮浓度（P<0.05）。因此，接种（）改善了苜蓿青贮饲料的发酵品质，抑制了腐败微生物的生长，并在不利的青贮条件下进一步延缓了苜蓿青贮饲料的变质。
