Zhao Shanshan, Yang Fengyuan, Wang Yuan, Fan Xiaomiao, Feng Changsong, Wang Yanping
Henan Key Laboratory of Ion Beam Bio-Engineering, College of Physics, Zhengzhou University, Zhengzhou 450000, China.
Henan Key Laboratory of Ion Beam Bio-Engineering, School of Agricultural Science, Zhengzhou University, Zhengzhou 450000, China.
Microorganisms. 2021 Jun 4;9(6):1225. doi: 10.3390/microorganisms9061225.
The aim of this study was to gain deeper insights into the dynamics of fermentation parameters and the bacterial community during the ensiling of high-moisture alfalfa. A commercial lactic acid bacteria (YX) inoculant was used as an additive. After 15 and 30 days of ensiling, the control silage (CK) exhibited a high pH and a high concentration of ammoniacal nitrogen (NH-N); and were the dominant genera. At 60 d, the pH value and the concentration of NH-N in CK silage increased compared with 15 and 30 d, propionic acid and butyric acid (BA) were detected, and had the highest abundance in the bacterial community. Compared with CK silage, inoculation of YX significantly promoted lactic acid and acetic acid accumulation and reduced pH and BA formation, did not significantly reduce the concentration of NH-N except at 60 d, and significantly promoted the abundance of and decreased the abundance of and , but did not significantly inhibit the growth of and . In conclusion, high-moisture alfalfa naturally ensiled is prone to rot. Adding YX can delay the process of silage spoilage by inhibiting the growth of undesirable microorganisms to a certain extent.
本研究的目的是更深入地了解高水分苜蓿青贮过程中发酵参数和细菌群落的动态变化。使用一种商业乳酸菌（YX）接种剂作为添加剂。青贮15天和30天后，对照青贮料（CK）表现出高pH值和高浓度的氨态氮（NH-N）； 是优势属。在60天时，CK青贮料的pH值和NH-N浓度与15天和30天时相比有所增加，检测到丙酸和丁酸（BA），并且 在细菌群落中的丰度最高。与CK青贮料相比，接种YX显著促进了乳酸和乙酸的积累，降低了pH值和BA的形成，除60天外，对NH-N浓度没有显著降低作用，显著促进了 的丰度，降低了 和 的丰度，但对 和 的生长没有显著抑制作用。总之，自然青贮的高水分苜蓿容易腐烂。添加YX可以通过在一定程度上抑制不良微生物的生长来延缓青贮料变质的过程。
