Microbial Food-Safety Research Unit, Department of Food Quality and Safety, The Volcani Center, Agriculture Research Organization, Institute for Postharvest and Food Sciences, Derech HaMaccabim Road 68, POB 15159, 7528809, Rishon-LeZion, Israel.
Appl Microbiol Biotechnol. 2018 May;102(9):4025-4037. doi: 10.1007/s00253-018-8903-y. Epub 2018 Mar 13.
Microbial population dynamics associated with corn silage, with and without Lactobacillus plantarum treatment, was studied. Whole crop corn was ensiled using laboratory silos and sampled at different times, up to 3 months. The dominant bacteria, before ensiling, were Acinetobacter (38.5%) and Klebsiella (16.3%), while the dominant fungi were Meyerozyma (53.5%) and Candida (27.7%). During ensiling, the microbial population shifted considerably, and Lactobacillus (> 94%) and Candida (> 74%) became the most dominant microbial genera in both treated and untreated silages. Yet, lactic acid content was higher in the treated silage, while the microbial diversity was lower than in the untreated silage. Upon aerobic exposure, spoilage occurred more rapidly in the treated silage, possibly due to the higher abundance of lactic acid-assimilating fungi, such as Candida. Our study is the first to describe microbial population dynamics during whole-crop corn ensiling and the results indicate that microbial diversity may be an indicator of aerobic stability.
本研究旨在探讨添加和不添加植物乳杆菌处理的全株玉米青贮过程中与微生物种群动态相关的问题。采用实验室青贮窖对全株玉米进行青贮，并在不同时间点进行采样，最长可达 3 个月。在青贮前，优势细菌为不动杆菌（38.5%）和克雷伯氏菌（16.3%），而优势真菌为迈耶罗酵母（53.5%）和假丝酵母（27.7%）。在青贮过程中，微生物种群发生了很大的变化，无论是在处理过的青贮料还是未处理的青贮料中，乳杆菌（>94%）和假丝酵母（>74%）都成为了最主要的微生物属。然而，处理过的青贮料中的乳酸含量更高，而微生物多样性则低于未处理的青贮料。在有氧暴露下，处理过的青贮料更容易发生变质，这可能是由于乳酸同化真菌（如假丝酵母）的丰度更高所致。本研究首次描述了全株玉米青贮过程中的微生物种群动态，结果表明微生物多样性可能是有氧稳定性的一个指标。
