Cremonesi Fausto, Bonfanti Stefano, Idda Antonella, Lange-Consiglio Anna
Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy.
Private Practitioner, Milan, Italy.
Front Vet Sci. 2020 Aug 13;7:517. doi: 10.3389/fvets.2020.00517. eCollection 2020.
Recent studies on cull cows have shown that ovarian abnormalities, particularly ovarian insufficiency, are the main cause of reproductive failure. The aim of this study was to treat bovine ovarian failure with intraovarian administration of autologous platelet rich plasma (PRP), which is rich in growth factors, chemokines, and cytokines that could stimulate follicular growth and steroidogenesis. Twelve cows with ovarian hypofunction were enrolled in the study and they were randomly allocated in control group (CTR) and treated group (six animal for group). In the treated group, only five animals received the PRP treatment because intraovarian administration was hindered in one by a rectovaginal fistula. Animals of control group were treated by intraovarian administration of physiological solution. In the 4 weeks after PRP injection, a mild to strong increase in progesterone (PRG) concentrations was detected in four of the five cows treated. Artificial insemination (AI) resulted in four pregnancies that are still ongoing (7th month). Intraovarian administration of PRP improved ovarian function after 2 months of treatment. This effect may be due to reduction of follicular atresia or to revitalization of dormant oocytes allowing restoration of fertility.
最近关于淘汰母牛的研究表明，卵巢异常，尤其是卵巢功能不全，是繁殖失败的主要原因。本研究的目的是通过卵巢内注射富含生长因子、趋化因子和细胞因子的自体富血小板血浆（PRP）来治疗牛卵巢功能衰竭，这些因子可刺激卵泡生长和类固醇生成。12头卵巢功能减退的母牛被纳入研究，并随机分为对照组（CTR）和治疗组（每组6头动物）。在治疗组中，只有5头动物接受了PRP治疗，因为其中1头因直肠阴道瘘而阻碍了卵巢内给药。对照组动物通过卵巢内注射生理溶液进行治疗。在注射PRP后的4周内，接受治疗的5头母牛中有4头检测到孕酮（PRG）浓度有轻度至显著升高。人工授精（AI）导致4头母牛怀孕，目前仍在继续（第7个月）。治疗2个月后，卵巢内注射PRP改善了卵巢功能。这种效果可能是由于卵泡闭锁减少或休眠卵母细胞的复苏，从而恢复了生育能力。
