小鼠巨噬细胞炎性蛋白（MIP）cDNA的克隆与特性分析，MIP是一种具有炎性和趋化特性的新型单核因子。
Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties.
作者信息
Davatelis G, Tekamp-Olson P, Wolpe S D, Hermsen K, Luedke C, Gallegos C, Coit D, Merryweather J, Cerami A
机构信息
Laboratory of Medical Biochemistry, Rockefeller University, New York, New York 10021.
出版信息
J Exp Med. 1988 Jun 1;167(6):1939-44. doi: 10.1084/jem.167.6.1939.
Abstract
In the course of studies on cachectin/TNF being conducted in our laboratory, a novel macrophage product has been detected and characterized. Termed macrophage inflammatory protein or MIP, this protein appears to be an endogenous mediator of the inflammatory events induced by endotoxin. A cDNA cloned probe for this protein has been isolated from a lambda gt10 phage library prepared from poly(A)+ RNA obtained of endotoxin-induced RAW264.7 cells. The sequence codes for a 92 amino acid-long polypeptide, of which 69 amino acids correspond to the mature product. The sequence predicts a molecular weight of 7,889 and structural analysis of the protein indicates a characteristic signal sequence alpha-helix and a hydrophobic core. Sequence data also confirm no sequence similarity to any other protein listed in the Dayhoff data base.
摘要
在我们实验室进行的关于恶病质素/肿瘤坏死因子（cachectin/TNF）的研究过程中，已检测并鉴定出一种新型巨噬细胞产物。这种蛋白质被称为巨噬细胞炎性蛋白或MIP，它似乎是内毒素诱导的炎症反应的内源性介质。已从由内毒素诱导的RAW264.7细胞获得的聚腺苷酸加尾RNA（poly(A)+ RNA）制备的λgt10噬菌体文库中分离出该蛋白质的cDNA克隆探针。该序列编码一个92个氨基酸长的多肽，其中69个氨基酸对应于成熟产物。该序列预测分子量为7889，对该蛋白质的结构分析表明其具有特征性的信号序列α螺旋和疏水核心。序列数据还证实与Dayhoff数据库中列出的任何其他蛋白质均无序列相似性。
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本文引用的文献
1
Construction and evaluation of an instrument for the automated synthesis of oligodeoxyribonucleotides.用于寡脱氧核糖核苷酸自动合成的仪器的构建与评估。
DNA. 1984 Oct;3(5):401-11. doi: 10.1089/dna.1984.3.401.
2
Cell-specific expression controlled by the 5'-flanking region of insulin and chymotrypsin genes.由胰岛素基因和胰凝乳蛋白酶基因的5'侧翼区域控制的细胞特异性表达。
Nature. 1983;306(5943):557-61. doi: 10.1038/306557a0.
3
A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides.真核生物和原核生物信号肽中一个假定的信号肽酶识别位点及序列。
J Mol Biol. 1983 Jun 25;167(2):391-409. doi: 10.1016/s0022-2836(83)80341-6.
4
A simple and very efficient method for generating cDNA libraries.一种简单且非常有效的生成cDNA文库的方法。
Gene. 1983 Nov;25(2-3):263-9. doi: 10.1016/0378-1119(83)90230-5.
5
Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.点突变确定了AUG起始密码子侧翼的一个序列，该序列可调节真核生物核糖体的翻译。
Cell. 1986 Jan 31;44(2):283-92. doi: 10.1016/0092-8674(86)90762-2.
6
An update on human interleukin-1: from molecular biology to clinical relevance.人类白细胞介素-1的最新进展：从分子生物学到临床相关性
J Clin Immunol. 1985 Sep;5(5):287-97. doi: 10.1007/BF00918247.
7
Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.在氯化铵中与碱基组成无关的杂交：一种用于高度复杂基因文库寡核苷酸筛选的方法。
Proc Natl Acad Sci U S A. 1985 Mar;82(6):1585-8. doi: 10.1073/pnas.82.6.1585.
8
Codon usage tabulated from the GenBank genetic sequence data.从GenBank基因序列数据中整理出的密码子使用情况。
Nucleic Acids Res. 1986;14 Suppl(Suppl):r151-97. doi: 10.1093/nar/14.suppl.r151.
9
Secretory products of macrophages.巨噬细胞的分泌产物。
J Clin Invest. 1987 Feb;79(2):319-26. doi: 10.1172/JCI112815.
10
Molecular cloning of a functional bovine interleukin 2 cDNA.功能性牛白细胞介素2 cDNA的分子克隆
Proc Natl Acad Sci U S A. 1986 May;83(10):3228-32. doi: 10.1073/pnas.83.10.3228.
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