Davatelis G, Tekamp-Olson P, Wolpe S D, Hermsen K, Luedke C, Gallegos C, Coit D, Merryweather J, Cerami A
Laboratory of Medical Biochemistry, Rockefeller University, New York, New York 10021.
J Exp Med. 1988 Jun 1;167(6):1939-44. doi: 10.1084/jem.167.6.1939.
In the course of studies on cachectin/TNF being conducted in our laboratory, a novel macrophage product has been detected and characterized. Termed macrophage inflammatory protein or MIP, this protein appears to be an endogenous mediator of the inflammatory events induced by endotoxin. A cDNA cloned probe for this protein has been isolated from a lambda gt10 phage library prepared from poly(A)+ RNA obtained of endotoxin-induced RAW264.7 cells. The sequence codes for a 92 amino acid-long polypeptide, of which 69 amino acids correspond to the mature product. The sequence predicts a molecular weight of 7,889 and structural analysis of the protein indicates a characteristic signal sequence alpha-helix and a hydrophobic core. Sequence data also confirm no sequence similarity to any other protein listed in the Dayhoff data base.
在我们实验室进行的关于恶病质素/肿瘤坏死因子（cachectin/TNF）的研究过程中，已检测并鉴定出一种新型巨噬细胞产物。这种蛋白质被称为巨噬细胞炎性蛋白或MIP，它似乎是内毒素诱导的炎症反应的内源性介质。已从由内毒素诱导的RAW264.7细胞获得的聚腺苷酸加尾RNA（poly(A)+ RNA）制备的λgt10噬菌体文库中分离出该蛋白质的cDNA克隆探针。该序列编码一个92个氨基酸长的多肽，其中69个氨基酸对应于成熟产物。该序列预测分子量为7889，对该蛋白质的结构分析表明其具有特征性的信号序列α螺旋和疏水核心。序列数据还证实与Dayhoff数据库中列出的任何其他蛋白质均无序列相似性。
