Tekamp-Olson P, Gallegos C, Bauer D, McClain J, Sherry B, Fabre M, van Deventer S, Cerami A
Chiron Corporation, Emeryville, California 94608.
J Exp Med. 1990 Sep 1;172(3):911-9. doi: 10.1084/jem.172.3.911.
A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and the nucleotide sequence determined. This cDNA was used to clone cDNAs for human homologues of MIP-2 from a library prepared from phorbol myristate acetate-treated and LPS-stimulated U937 cells. Two homologues were isolated and sequenced. Human MIP-2 alpha and MIP-2 beta are highly homologous to each other and to a previously isolated gene, human gro/melanoma growth-stimulating activity (MGSA). These three human genes, MIP-2 alpha, MIP-2 beta, and gro/MGSA, constitute a sub-family within the cytokine family represented by platelet factor 4 and interleukin 8.
从小鼠巨噬细胞炎性蛋白2（MIP - 2）的cDNA克隆已从用脂多糖（LPS）刺激的RAW 264.7细胞构建的文库中分离出来，并测定了核苷酸序列。该cDNA用于从佛波酯肉豆蔻酸酯乙酸盐处理且LPS刺激的U937细胞构建的文库中克隆MIP - 2人类同源物的cDNA。分离并测序了两个同源物。人类MIP - 2α和MIP - 2β彼此高度同源，并且与先前分离的基因人类生长调节致癌基因/黑素瘤生长刺激活性（MGSA）高度同源。这三个人类基因，MIP - 2α、MIP - 2β和生长调节致癌基因/MGSA，在以血小板因子4和白细胞介素8为代表的细胞因子家族中构成一个亚家族。
